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Literature summary extracted from

  • Albi, T.; Ruiz, M.T.; de Los Reyes, P.; Valverde, F.; Romero, J.M.
    Characterization of the sucrose phosphate phosphatase (SPP) isoforms from Arabidopsis thaliana and role of the S6PPc domain in dimerization (2016), PLoS ONE, 11, e0166308 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.24 expression in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.24 additional information fusion of the catalytic domain of isoform Spp2 to the C-terminus of the monomeric SPP from Synechocystis sp. the catalytic domain is responsible of protein dimerization Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.24 0.73
-
 sucrose 6-phosphate pH 7.0, 37°C Arabidopsis thaliana
3.1.3.24 3.5
-
 sucrose 6-phosphate pH 7.0, 37°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.24 Mg2+ maximal activity is observed in the presence of Mg2+ Arabidopsis thaliana
3.1.3.24 Mg2+ maximal activity is observed in the presence of Mg2+, optimum concentration 5 mM Arabidopsis thaliana
3.1.3.24 Mn2+ about 30% of the activity with Mg2+ Arabidopsis thaliana
3.1.3.24 additional information divalent cation is required Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.24 90000
-
 gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.24 Arabidopsis thaliana Q93XN8 isoform Spp3b
-
3.1.3.24 Arabidopsis thaliana Q9SJ66
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.24 additional information highest level of expression among the aerial parts of the plant Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.24 sucrose 6-phosphate + H2O
-
 Arabidopsis thaliana sucrose + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.24 dimer 2 * 52000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.1.3.24 SPP2
-
 Arabidopsis thaliana
3.1.3.24 Spp3b
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.24 3.6
-
 sucrose 6-phosphate pH 7.0, 37°C Arabidopsis thaliana
3.1.3.24 67.8
-
 sucrose 6-phosphate pH 7.0, 37°C Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.24 1
-
 sucrose 6-phosphate pH 7.0, 37°C Arabidopsis thaliana
3.1.3.24 92.5
-
 sucrose 6-phosphate pH 7.0, 37°C Arabidopsis thaliana