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Literature summary extracted from

  • Kobayashi, T.; Sasaki, S.; Utsumi, Y.; Fujita, N.; Umeda, K.; Sawada, T.; Kubo, A.; Abe, J.; Colleoni, C.; Ball, S.; Nakamura, Y.
    Comparison of chain-length preferences and glucan specificities of isoamylase-type alpha-glucan debranching enzymes from rice, Cyanobacteria, and Bacteria (2016), PLoS ONE, 11, e0157020 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.68 expressed in Escherichia coli BL21 cells Escherichia coli
3.2.1.68 expressed in Escherichia coli BL21 cells Oryza sativa
3.2.1.68 expressed in Escherichia coli BL21 cells Pseudomonas amyloderamosa
3.2.1.68 expressed in Escherichia coli BL21 cells Synechococcus elongatus
3.2.1.68 expressed in Escherichia coli BL21 cells Crocosphaera subtropica ATCC 51142

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.68 Crocosphaera subtropica ATCC 51142
-
-
-
3.2.1.68 Escherichia coli
-
-
-
3.2.1.68 Oryza sativa
-
-
-
3.2.1.68 Pseudomonas amyloderamosa
-
-
-
3.2.1.68 Synechococcus elongatus
-
-
-
3.2.1.68 Synechococcus elongatus PCC7942
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.68 HitrapQ column chromatography and TSKgel DEAE-5PW column chromatography Escherichia coli
3.2.1.68 HitrapQ column chromatography and TSKgel DEAE-5PW column chromatography Oryza sativa
3.2.1.68 HitrapQ column chromatography and TSKgel DEAE-5PW column chromatography Pseudomonas amyloderamosa
3.2.1.68 HitrapQ column chromatography and TSKgel DEAE-5PW column chromatography Synechococcus elongatus
3.2.1.68 HitrapQ column chromatography and TSKgel DEAE-5PW column chromatography Crocosphaera subtropica ATCC 51142

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.68 endosperm
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.68 amylopectin + H2O isoform ISA1 removes the wide range of chains with a degree of polymerization (DP) of 6-20 from amylopectin although the long chains of DP higher than 20 are debranched by the prolonged incubation. Isoform ISA1 shows 356% activity compared to phosphorylase a limit dextrin. Isoform ISA3 shows trace activity compared to phosphorylase a limit dextrin Oryza sativa ?
-
 ?
3.2.1.68 amylopectin + H2O the enzyme can debranch almost all of component chains of amylopectin Pseudomonas amyloderamosa ?
-
 ?
3.2.1.68 amylopectin + H2O the enzyme can debranch almost all of component chains of amylopectin and shows 9.4% activity compared to phosphorylase a limit dextrin Crocosphaera subtropica ATCC 51142 ?
-
 ?
3.2.1.68 amylopectin + H2O the enzyme shows 0.8% activity compared to phosphorylase a limit dextrin Escherichia coli ?
-
 ?
3.2.1.68 beta-amylase limit dextrin + H2O isoform ISA1 shows 71% activity compared to phosphorylase a limit dextrin. Isoform ISA3 shows 48% activity compared to phosphorylase a limit dextrin Oryza sativa ?
-
 ?
3.2.1.68 beta-amylase limit dextrin + H2O the enzyme shows 34% activity compared to phosphorylase a limit dextrin Escherichia coli ?
-
 ?
3.2.1.68 beta-amylase limit dextrin + H2O the enzyme shows 55% activity compared to phosphorylase a limit dextrin Crocosphaera subtropica ATCC 51142 ?
-
 ?
3.2.1.68 oyster glycogen + H2O isoform ISA1 shows 104% activity compared to phosphorylase a limit dextrin. Isoform ISA3 shows 1.5% activity compared to phosphorylase a limit dextrin Oryza sativa ?
-
 ?
3.2.1.68 oyster glycogen + H2O the enzyme shows 14% activity compared to phosphorylase a limit dextrin Crocosphaera subtropica ATCC 51142 ?
-
 ?
3.2.1.68 oyster glycogen + H2O the enzyme shows 2% activity compared to phosphorylase a limit dextrin Escherichia coli ?
-
 ?
3.2.1.68 phosphorylase a limit dextrin + H2O 100% activity Escherichia coli ?
-
 ?
3.2.1.68 phosphorylase a limit dextrin + H2O 100% activity Oryza sativa ?
-
 ?
3.2.1.68 phosphorylase a limit dextrin + H2O 100% activity Crocosphaera subtropica ATCC 51142 ?
-
 ?
3.2.1.68 phytoglycogen + H2O short chains of DP3 and DP4 are most effectively liberated by isoform ISA1 during the short period, and with the elapse of the incubation time intermediate chains with the peak of DP6 are more significantly produced. Almost only short chains of DP3 and DP4 are found after the isoform ISA3 reaction for 5-10 min, then during the course of enzymatic reaction until 60 min a small amount of the DP5-10 chains is found Oryza sativa ?
-
 ?
3.2.1.68 phytoglycogen + H2O the enzyme can debranch almost all of component chains of phytoglycogen Pseudomonas amyloderamosa ?
-
 ?
3.2.1.68 phytoglycogen + H2O the enzyme removes only exclusively chains of DP3 and DP4 when incubated with phytoglycogen while DP5-7 chains are slightly liberated after the prolonged incubation Escherichia coli ?
-
 ?
3.2.1.68 phytoglycogen + H2O the liberated chains formed two distinct peaks: the first large peak of DP3-4 and the second small peak of DP5-10 Crocosphaera subtropica ATCC 51142 ?
-
 ?
3.2.1.68 phytoglycogen + H2O the liberated chains from phytoglycogen are enriched in short chains of DP3-6, but contained longer chains of DP higer than 7, decreasing in amounts with the increase in chain-length until DP about 15 Synechococcus elongatus ?
-
 ?
3.2.1.68 phytoglycogen + H2O the liberated chains from phytoglycogen are enriched in short chains of DP3-6, but contained longer chains of DP higer than 7, decreasing in amounts with the increase in chain-length until DP about 15 Synechococcus elongatus PCC7942 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.68 GlgX
-
 Escherichia coli
3.2.1.68 ISA
-
 Synechococcus elongatus
3.2.1.68 ISA
-
 Crocosphaera subtropica ATCC 51142
3.2.1.68 ISA1
-
 Oryza sativa
3.2.1.68 ISA1
-
 Pseudomonas amyloderamosa
3.2.1.68 ISA3
-
 Oryza sativa