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Literature summary extracted from

  • Chang, C.T.; Lin, Y.L.; Lu, S.W.; Huang, C.W.; Wang, Y.T.; Chung, Y.C.
    Characterization of a chitosanase from Jelly Fig (Ficus awkeotsang Makino) latex and its application in the production of water-soluble low molecular weight chitosans (2016), PLoS ONE, 11, e0150490 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.132 additional information
-
 chitosan Km-value: 0.089 mg/ml, 50°C, pH 4.5 Ficus pumila var. awkeotsang

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 20500
-
 SDS-PAGE Ficus pumila var. awkeotsang

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Ficus pumila var. awkeotsang
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Ficus pumila var. awkeotsang

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.132 latex
-
 Ficus pumila var. awkeotsang
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 aminoethyl chitosan + H2O average molecular weight of aminoethyl low molecular weight chitosan is 8.89 kDa Ficus pumila var. awkeotsang aminoethyl low molecular weight chitosan + ?
-
 ?
3.2.1.132 carboxymethyl chitosan + H2O average molecular weight of carboxymethyl low molecular weight chitosan is 11.2 kDa Ficus pumila var. awkeotsang carboxymethyl low molecular weight chitosan + ?
-
 ?
3.2.1.132 chitosan + H2O the enzyme shows activity toward chitosan polymers which exhibit various degrees of deacetylation (21-94%). It hydrolyzes 70-84% deacetylated chitosan polymers most effectively. Substrate specificity analysis indicates that the enzyme catalyzes the hydrolysis of chitin and chitosan polymers and their derivatives Ficus pumila var. awkeotsang ?
-
 ?
3.2.1.132 ethylene glycol chitosan + H2O average molecular weight of ethylene glycol low molecular weight chitosan is 11.2 kDa Ficus pumila var. awkeotsang ethylene glycol low molecular weight chitosan + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 20500, SDS-PAGE Ficus pumila var. awkeotsang

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 50
-
-
 Ficus pumila var. awkeotsang

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 4.5
-
 substrate: chitosan Ficus pumila var. awkeotsang

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.132 Ficus pumila var. awkeotsang estimated by isoelectric focusing electrophoresis on Phast-Gel IEF 3-9
-
 3.5