BRENDA - Enzyme Database

Cloning and characterization of three Eimeria tenella lipid phosphate phosphatases

Guo, A.; Cai, J.; Luo, X.; Zhang, S.; Hou, J.; Li, H.; Cai, X.; PLoS ONE 10, e122736 (2015)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
expression of His6-tagged EtLPP1 in HEK293 cells produces immunoreactive proteins with variable molecular sizes, suggesting the presence of multiple forms of enzyme; expression of His6-tagged EtLPP1 in HEK293 cells produces immunoreactive proteins with variable molecular sizes, suggesting the presence of multiple forms of enzyme; expression of His6-tagged EtLPP1 in HEK293 cells produces immunoreactive proteins with variable molecular sizes, suggesting the presence of multiple forms of enzyme
Eimeria tenella
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
additional information
the enzyme is insensitive to N-ethylmaleimide; the enzyme is insensitive to N-ethylmaleimide; the enzyme is insensitive to N-ethylmaleimide
Eimeria tenella
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.022
-
ceramide-1-phosphate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.028
-
sphingosine-1-phosphate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.046
-
lysophosphatidate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.052
-
ceramide-1-phosphate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.083
-
lysophosphatidate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.179
-
lysophosphatidate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.237
-
sphingosine-1-phosphate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.26
-
sphingosine-1-phosphate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.284
-
phosphatidate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.355
-
phosphatidate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.369
-
phosphatidate
pH 6.5, 37C
Eimeria tenella
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
37000
-
and 60000 Da and 110000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP3
Eimeria tenella
3.1.3.4
40000
-
and 42000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP1; and 70000 Da and 120000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP2
Eimeria tenella
3.1.3.4
42000
-
and 40000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP1
Eimeria tenella
3.1.3.4
60000
-
and 37000 Da and 110000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP3
Eimeria tenella
3.1.3.4
70000
-
and 40000 Da and 120000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP2
Eimeria tenella
3.1.3.4
110000
-
and 60000 Da and 37000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP3
Eimeria tenella
3.1.3.4
120000
-
and 40000 da and 70000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP2
Eimeria tenella
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Eimeria tenella
A0A0A7H6F4
-
-
3.1.3.4
Eimeria tenella
A0A0A7H9C0
-
-
3.1.3.4
Eimeria tenella
A0A0A7H9U0
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.3.4
glycoprotein
the enzyme is partially N-glycosylated; the enzyme is partially N-glycosylated; the enzyme is partially N-glycosylated
Eimeria tenella
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
sporozoite
particularly high expression levels of the three lipid phosphate phosphatase (EtLPP) genes are detected in the sporozoite stage of the Eimeria tenella life cycle, suggesting that their encoded proteins might play an important biological function in the sporozoite stage; particularly high expression levels of the three lipid phosphate phosphatase (EtLPP) genes are detected in the sporozoite stage of the Eimeria tenella life cycle, suggesting that their encoded proteins might play an important biological function in the sporozoite stage; particularly high expression levels of the three lipid phosphate phosphatases (EtLPP) genes are detected in the sporozoite stage of the Eimeria tenella life cycle, suggesting that their encoded proteins might play an important biological function in the sporozoite stage
Eimeria tenella
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
ceramide-1-phosphate + H2O
-
751935
Eimeria tenella
ceramide + phosphate
-
-
-
?
3.1.3.4
lysophosphatidate + H2O
-
751935
Eimeria tenella
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidate + H2O
-
751935
Eimeria tenella
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine-1-phosphate + H2O
-
751935
Eimeria tenella
sphingosine + phosphate
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.3.4
37
-
assay at; assay at; assay at
Eimeria tenella
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6.5
-
assay at; assay at; assay at
Eimeria tenella
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
expression of His6-tagged EtLPP1 in HEK293 cells produces immunoreactive proteins with variable molecular sizes, suggesting the presence of multiple forms of enzyme
Eimeria tenella
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
additional information
the enzyme is insensitive to N-ethylmaleimide
Eimeria tenella
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.022
-
ceramide-1-phosphate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.028
-
sphingosine-1-phosphate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.046
-
lysophosphatidate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.052
-
ceramide-1-phosphate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.083
-
lysophosphatidate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.179
-
lysophosphatidate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.237
-
sphingosine-1-phosphate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.26
-
sphingosine-1-phosphate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.284
-
phosphatidate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.355
-
phosphatidate
pH 6.5, 37C
Eimeria tenella
3.1.3.4
0.369
-
phosphatidate
pH 6.5, 37C
Eimeria tenella
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
37000
-
and 60000 Da and 110000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP3
Eimeria tenella
3.1.3.4
40000
-
and 42000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP1
Eimeria tenella
3.1.3.4
40000
-
and 70000 Da and 120000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP2
Eimeria tenella
3.1.3.4
42000
-
and 40000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP1
Eimeria tenella
3.1.3.4
60000
-
and 37000 Da and 110000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP3
Eimeria tenella
3.1.3.4
70000
-
and 40000 Da and 120000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP2
Eimeria tenella
3.1.3.4
110000
-
and 60000 Da and 37000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP3
Eimeria tenella
3.1.3.4
120000
-
and 40000 da and 70000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP2
Eimeria tenella
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.3.4
glycoprotein
the enzyme is partially N-glycosylated
Eimeria tenella
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
sporozoite
particularly high expression levels of the three lipid phosphate phosphatase (EtLPP) genes are detected in the sporozoite stage of the Eimeria tenella life cycle, suggesting that their encoded proteins might play an important biological function in the sporozoite stage
Eimeria tenella
-
3.1.3.4
sporozoite
particularly high expression levels of the three lipid phosphate phosphatases (EtLPP) genes are detected in the sporozoite stage of the Eimeria tenella life cycle, suggesting that their encoded proteins might play an important biological function in the sporozoite stage
Eimeria tenella
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
ceramide-1-phosphate + H2O
-
751935
Eimeria tenella
ceramide + phosphate
-
-
-
?
3.1.3.4
lysophosphatidate + H2O
-
751935
Eimeria tenella
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidate + H2O
-
751935
Eimeria tenella
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine-1-phosphate + H2O
-
751935
Eimeria tenella
sphingosine + phosphate
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.3.4
37
-
assay at
Eimeria tenella
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6.5
-
assay at
Eimeria tenella