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Literature summary extracted from

  • Mano, J.; Ishibashi, A.; Muneuchi, H.; Morita, C.; Sakai, H.; Biswas, M.S.; Koeduka, T.; Kitajima, S.
    Acrolein-detoxifying isozymes of glutathione transferase in plants (2017), Planta, 245, 255-264 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli Rosetta2(DE3)pLysS cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.0304
-
 acrolein at pH 6.0 and 25°C Arabidopsis thaliana
2.5.1.18 0.0512
-
 glutathione at pH 6.0 and 25°C Arabidopsis thaliana
2.5.1.18 0.0709
-
 glutathione at pH 6.0 and 25°C Spinacia oleracea
2.5.1.18 0.093
-
 acrolein at pH 6.0 and 25°C Spinacia oleracea
2.5.1.18 0.395
-
 4-hydroxy-(E)-2-nonenal at pH 6.0 and 25°C Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Arabidopsis thaliana Q9ZRW8 ecotype Columbia-0
-
2.5.1.18 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 GSTrap 4B column chromatography, HiTrap Q column chromatography, and DEAE-Sepharose column chromatography Spinacia oleracea
2.5.1.18 GSTrap 4B column chromatography, HiTrap Q column chromatography, and DEAE-Sepharose column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 leaf
-
 Spinacia oleracea
-
2.5.1.18 leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 0.01
-
 less than 0.01 micromol/min/mg with 0.1 mM 4-nitrobenzyl chloride as substrate, at pH 6.0 and 25°C Spinacia oleracea
2.5.1.18 0.01
-
 less than 0.01 micromol/min/mg with 0.1 mM benzyl isothiocyanate as substrate, at pH 6.0 and 25°C Spinacia oleracea
2.5.1.18 0.01
-
 less than 0.01 micromol/min/mg with 0.1 mM crotonaldehyde as substrate, at pH 6.0 and 25°C Spinacia oleracea
2.5.1.18 0.05
-
 purified protein with poly(His) tag, with 12-oxo-phytodienoic acid as substrate, at pH 6.0 and 25°C Arabidopsis thaliana
2.5.1.18 0.07
-
 with 0.1 mM 4-hydroxy-(E)-2-nonenal as substrate, at pH 6.0 and 25°C Spinacia oleracea
2.5.1.18 1.7
-
 with 0.1 mM acrolein as substrate, at pH 6.0 and 25°C Spinacia oleracea
2.5.1.18 3.83
-
 purified protein with poly(His) tag, with acrolein as substrate, at pH 6.0 and 25°C Arabidopsis thaliana
2.5.1.18 5.2
-
 with 0.1 mM 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.0 and 25°C Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
 Spinacia oleracea S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 12-oxo-phytodienoic acid + glutathione
-
 Arabidopsis thaliana ?
-
 ?
2.5.1.18 4-hydroxy-(E)-2-nonenal + glutathione
-
 Spinacia oleracea ?
-
 ?
2.5.1.18 4-nitrobenzyl chloride + glutathione
-
 Spinacia oleracea S-(4-nitrobenzyl)glutathione + HCl
-
 ?
2.5.1.18 acrolein + glutathione
-
 Spinacia oleracea ?
-
 ?
2.5.1.18 acrolein + glutathione
-
 Arabidopsis thaliana ?
-
 ?
2.5.1.18 benzyl isothiocyanate + glutathione
-
 Spinacia oleracea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 ? x * 24000, SDS-PAGE Spinacia oleracea
2.5.1.18 ? x * 29000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GST
-
 Spinacia oleracea
2.5.1.18 GST Tau19
-
 Arabidopsis thaliana
2.5.1.18 GST-Acr isozyme Spinacia oleracea
2.5.1.18 GSTU19
-
 Arabidopsis thaliana
2.5.1.18 RX:glutathione R-transferase
-
 Spinacia oleracea
2.5.1.18 RX:glutathione R-transferase
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.0709
-
 glutathione at pH 6.0 and 25°C Spinacia oleracea
2.5.1.18 0.262
-
 4-hydroxy-(E)-2-nonenal at pH 6.0 and 25°C Spinacia oleracea
2.5.1.18 1.15
-
 acrolein at pH 6.0 and 25°C Spinacia oleracea
2.5.1.18 1.78
-
 acrolein at pH 6.0 and 25°C Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.18 Spinacia oleracea isoelectric focusing
-
 5.9

General Information

EC Number General Information Comment Organism
2.5.1.18 metabolism the enzyme keeps the acrolein level low in plant cells Spinacia oleracea
2.5.1.18 metabolism the enzyme keeps the acrolein level low in plant cells Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 0.0709
-
 glutathione at pH 6.0 and 25°C Spinacia oleracea
2.5.1.18 0.664
-
 4-hydroxy-(E)-2-nonenal at pH 6.0 and 25°C Spinacia oleracea
2.5.1.18 12.3
-
 acrolein at pH 6.0 and 25°C Spinacia oleracea
2.5.1.18 59.3
-
 acrolein at pH 6.0 and 25°C Arabidopsis thaliana