Any feedback?
Literature summary extracted from
- Acyl carrier proteins from sunflower (Helianthus annuus L.) seeds and their influence on FatA and FatB acyl-ACP thioesterase activities (2016), Planta, 244, 479-490 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.2.14 | expressed in Escherichia coli | Helianthus annuus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.2.14 | 0.00084 | - |
palmitoyl-[acyl-carrier protein] | isoform FatB, palmitoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus |  |
| 3.1.2.14 | 0.0009 | - |
stearoyl-[acyl-carrier protein] | isoform FatA, stearoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.0009 | 0.002 | oleoyl-[acyl-carrier protein] | isoform FatB, oleoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.0011 | - |
stearoyl-[acyl-carrier protein] | isoform FatB, stearoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.0013 | - |
palmitoyl-[acyl-carrier protein] | isoform FatA, palmitoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.0013 | - |
oleoyl-[acyl-carrier protein] | isoform FatB, oleoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.0021 | - |
oleoyl-[acyl-carrier protein] | isoform FatA, oleoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.0021 | 0.0028 | oleoyl-[acyl-carrier protein] | isoform FatA, oleoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.004 | 0.0051 | palmitoyl-[acyl-carrier protein] | isoform FatB, palmitoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.0042 | 0.0049 | stearoyl-[acyl-carrier protein] | isoform FatA, stearoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.0045 | 0.0051 | palmitoyl-[acyl-carrier protein] | isoform FatA, palmitoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.0067 | 0.0128 | stearoyl-[acyl-carrier protein] | isoform FatB, stearoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.2.14 | Helianthus annuus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.2.14 | Ni-NTA column chromatography | Helianthus annuus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.2.14 | oleoyl-[acyl-carrier protein] + H2O | best substrate for isoform FatA | Helianthus annuus | [acyl-carrier protein] + oleate | - |
? | |
| 3.1.2.14 | palmitoyl-[acyl-carrier protein] + H2O | best substrate for isoform FatB | Helianthus annuus | [acyl-carrier protein] + palmitate | - |
? | |
| 3.1.2.14 | stearoyl-[acyl-carrier protein] + H2O | - |
Helianthus annuus | [acyl-carrier protein] + stearate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.2.14 | acyl-ACP thioesterase | - |
Helianthus annuus |
| 3.1.2.14 | FatA | - |
Helianthus annuus |
| 3.1.2.14 | FatB | - |
Helianthus annuus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.2.14 | 0.18 | 0.7 | oleoyl-[acyl-carrier protein] | isoform FatB, oleoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.27 | - |
stearoyl-[acyl-carrier protein] | isoform FatB, stearoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.4 | - |
stearoyl-[acyl-carrier protein] | isoform FatA, stearoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.5 | - |
palmitoyl-[acyl-carrier protein] | isoform FatA, palmitoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 0.52 | - |
palmitoyl-[acyl-carrier protein] | isoform FatB, palmitoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 1.19 | - |
oleoyl-[acyl-carrier protein] | isoform FatB, oleoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 3.5 | 4.2 | palmitoyl-[acyl-carrier protein] | isoform FatA, palmitoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 3.66 | - |
stearoyl-[acyl-carrier protein] | isoform FatA, stearoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 9.4 | 9.6 | palmitoyl-[acyl-carrier protein] | isoform FatB, palmitoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 11.4 | - |
oleoyl-[acyl-carrier protein] | isoform FatA, oleoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 17.3 | 25.5 | oleoyl-[acyl-carrier protein] | isoform FatA, oleoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 20.4 | 26.5 | stearoyl-[acyl-carrier protein] | isoform FatB, stearoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.2.14 | 90 | 850 | oleoyl-[acyl-carrier protein] | isoform FatB, oleoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 240 | - |
stearoyl-[acyl-carrier protein] | isoform FatB, stearoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 400 | - |
palmitoyl-[acyl-carrier protein] | isoform FatA, palmitoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 400 | - |
stearoyl-[acyl-carrier protein] | isoform FatA, stearoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 620 | - |
palmitoyl-[acyl-carrier protein] | isoform FatB, palmitoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 760 | 850 | palmitoyl-[acyl-carrier protein] | isoform FatA, palmitoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 810 | 1300 | stearoyl-[acyl-carrier protein] | isoform FatA, stearoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 910 | - |
oleoyl-[acyl-carrier protein] | isoform FatB, oleoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 1900 | 2400 | palmitoyl-[acyl-carrier protein] | isoform FatB, palmitoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 2100 | 3200 | stearoyl-[acyl-carrier protein] | isoform FatB, stearoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 5400 | - |
oleoyl-[acyl-carrier protein] | isoform FatA, oleoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.1.2.14 | 7400 | 12300 | oleoyl-[acyl-carrier protein] | isoform FatA, oleoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
