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Literature summary extracted from

  • Nobre, A.; Empadinhas, N.; Nobre, M.; Lourenco, E.; Maycock, C.; Ventura, M.; Mingote, A.; da Costa, M.
    The plant Selaginella moellendorffii possesses enzymes for synthesis and hydrolysis of the compatible solutes mannosylglycerate and glucosylglycerate (2013), Planta, 237, 891-901 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.208 expression in Escherichia coli Selaginella moellendorffii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.208 additional information not inhibitory: EDTA Selaginella moellendorffii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.208 2.9
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate pH 6.0, 25°C Selaginella moellendorffii
3.2.1.208 5.9
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate pH 6.0, 40°C Selaginella moellendorffii
3.2.1.208 11.8
-
 2-O-(alpha-D-mannopyranosyl)-D-glycerate pH 6.0, 40°C Selaginella moellendorffii
3.2.1.208 21.4
-
 2-O-(alpha-D-mannopyranosyl)-D-glycerate pH 6.0, 25°C Selaginella moellendorffii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.208 Ca2+ activation by 10 mM cation in decreasing order of efficiency is Mn2+, Ca2+, Mg2+ Selaginella moellendorffii
3.2.1.208 Mg2+ activation by 10 mM cation in decreasing order of efficiency is Mn2+, Ca2+, Mg2+ Selaginella moellendorffii
3.2.1.208 Mn2+ maximum activity in presence of 10 mM Mn2+. Activation by 10 mM cation in decreasing order of efficiency is Mn2+, Ca2+, Mg2+ Selaginella moellendorffii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.208 55300
-
 gel filtration Selaginella moellendorffii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.208 Selaginella moellendorffii D8QTR2
-
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.208 0°C, stable for more than 2 months, stable upon freeze/Thawing Selaginella moellendorffii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.208 2-O-(alpha-D-glucopyranosyl)-D-glycerate + H2O
-
 Selaginella moellendorffii D-glucopyranose + D-glycerate
-
 ir
3.2.1.208 2-O-(alpha-D-mannopyranosyl)-D-glycerate + H2O
-
 Selaginella moellendorffii D-mannopyranose + D-glycerate
-
 ir

Subunits

EC Number Subunits Comment Organism
3.2.1.208 monomer 1 * 54500, calculated from sequence Selaginella moellendorffii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.208 SELMODRAFT_437793
-
 Selaginella moellendorffii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.208 40
-
-
 Selaginella moellendorffii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.208 20 60
-
 Selaginella moellendorffii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.208 6 6.5
-
 Selaginella moellendorffii