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Literature summary extracted from

  • Cheng, X.X.; Zhao, L.H.; Klosterman, S.J.; Feng, H.J.; Feng, Z.L.; Wei, F.; Shi, Y.Q.; Li, Z.F.; Zhu, H.Q.
    The endochitinase VDECH from Verticillium dahliae inhibits spore germination and activates plant defense responses (2017), Plant Sci., 259, 12-23 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expressed in Escherichia coli BL21 cells Verticillium dahliae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Verticillium dahliae
-
 ?
-
 ?
3.2.1.14 chitin + H2O Verticillium dahliae Vd080
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Verticillium dahliae A0A286LWZ1
-
-
3.2.1.14 Verticillium dahliae Vd080 A0A286LWZ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 His-tagged purification Miniprep kit Verticillium dahliae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Verticillium dahliae ?
-
 ?
3.2.1.14 chitin + H2O
-
 Verticillium dahliae Vd080 ?
-
 ?
3.2.1.14 chitohexaose + H2O
-
 Verticillium dahliae chitobiose + chitotetraose + chitotriose
-
 ?
3.2.1.14 chitohexaose + H2O
-
 Verticillium dahliae Vd080 chitobiose + chitotetraose + chitotriose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 114000, His-tagged enzyme, SDS-PAGE Verticillium dahliae
3.2.1.14 ? x * 62200, His-tagged enzyme, calculated from amino acid sequence Verticillium dahliae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ECH
-
 Verticillium dahliae
3.2.1.14 endochitinase
-
 Verticillium dahliae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
-
 Verticillium dahliae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 100
-
 enzyme activity is retained after 15 min at 100°C Verticillium dahliae

General Information

EC Number General Information Comment Organism
3.2.1.14 physiological function the enzyme inhibits spore germination and activates plant defense responses Verticillium dahliae