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Literature summary extracted from

  • Lam, P.Y.; Zhu, F.Y.; Chan, W.L.; Liu, H.; Lo, C.
    Cytochrome P450 93G1 is a flavone synthase II that channels flavanones to the biosynthesis of tricin O-linked conjugates in rice (2014), Plant Physiol., 165, 1315-1327 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.76 CYP93G1 is placed under the control of the cauliflower mosaic virus 35S promoter and transformed into the Arabidopsis transparent testa6 (tt6) mutant. Transgenic expression of CYP93G1 in Arabidopsis thaliana results in the accumulation of different flavone O-glycosides, which are not naturally present in cruciferous plants Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.76 0.0015
-
 eriodictyol pH 8.0, 30°C Oryza sativa
1.14.19.76 0.0032
-
 naringenin pH 8.0, 30°C Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.76 eriodictyol + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa the enzyme channels flavanones to the biosynthesis of tricin O-linked conjugates luteolin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.19.76 naringenin + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa the enzyme channels flavanones to the biosynthesis of tricin O-linked conjugates apigenin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.76 Oryza sativa Q0JFI2 subsp. japonica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.76 eriodictyol + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa luteolin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.19.76 eriodictyol + [reduced NADPH-hemoprotein reductase] + O2 the enzyme channels flavanones to the biosynthesis of tricin O-linked conjugates Oryza sativa luteolin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.19.76 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa apigenin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.19.76 naringenin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme channels flavanones to the biosynthesis of tricin O-linked conjugates Oryza sativa apigenin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.76 CYP93G2
-
 Oryza sativa
1.14.19.76 cytochrome P450 93G1
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.76 30
-
 substrate: naringenin Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.19.76 8
-
 substrate: naringenin Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.76 cytochrome P-450 heme-thiolate protein Oryza sativa

General Information

EC Number General Information Comment Organism
1.14.19.76 metabolism the enzyme channels flavanones to the biosynthesis of tricin O-linked conjugates Oryza sativa