EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.364 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells and in Saccharomyces cerevisiae | Panax ginseng |
2.4.1.365 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells and in Saccharomyces cerevisiae | Panax ginseng |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.364 | 0.025 | - |
(20S)-protopanaxadiol | at pH 8.0 and 35°C | Panax ginseng | |
2.4.1.364 | 0.04 | - |
ginsenoside C-K | at pH 8.0 and 35°C | Panax ginseng | |
2.4.1.365 | 0.188 | - |
ginsenoside F2 | at pH 8.0 and 35°C | Panax ginseng | |
2.4.1.365 | 0.311 | - |
(20S)-ginsenoside Rh2 | at pH 8.0 and 35°C | Panax ginseng |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.364 | UDP-alpha-D-glucose + (20S)-protopanaxadiol | Panax ginseng | - |
UDP + (20S)-ginsenoside Rh2 | - |
? | |
2.4.1.364 | UDP-alpha-D-glucose + ginsenoside C-K | Panax ginseng | - |
UDP + ginsenoside F2 | - |
? | |
2.4.1.365 | UDP-alpha-D-glucose + (20S)-ginsenoside Rh2 | Panax ginseng | - |
UDP + (20S)-ginsenoside Rg3 | - |
? | |
2.4.1.365 | UDP-alpha-D-glucose + ginsenoside F2 | Panax ginseng | - |
UDP + ginsenoside Rd | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.364 | Panax ginseng | A0A0A6ZFR4 | - |
- |
2.4.1.365 | Panax ginseng | A0A0A6ZFY4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.364 | glutathione-Sepharose column chromatography | Panax ginseng |
2.4.1.365 | glutathione-Sepharose column chromatography | Panax ginseng |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.364 | leaf | - |
Panax ginseng | - |
2.4.1.364 | root | highest expression | Panax ginseng | - |
2.4.1.365 | leaf | - |
Panax ginseng | - |
2.4.1.365 | root | highest expression | Panax ginseng | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.364 | additional information | the enzyme glucosylates the C3 position of both (20S)-protopanaxadiol and ginsenoside C-K, but not C20 or C12 positions and also does not glucosylate hydroxyl residues at C3, C6, C12 or C20 positions in any protopanaxadiol-type ginsenoside | Panax ginseng | ? | - |
? | |
2.4.1.364 | UDP-alpha-D-glucose + (20S)-protopanaxadiol | - |
Panax ginseng | UDP + (20S)-ginsenoside Rh2 | - |
? | |
2.4.1.364 | UDP-alpha-D-glucose + ginsenoside C-K | - |
Panax ginseng | UDP + ginsenoside F2 | - |
? | |
2.4.1.365 | additional information | the enzyme glucosylates the C3-O-glucoside of both (20S)-ginsenoside Rh2 and ginsenoside F2, but not the C20-O-glucoside of ginsenoside C-K and ginsenoside F2, also displaying strong substrate specificity and regioselectivity | Panax ginseng | ? | - |
? | |
2.4.1.365 | UDP-alpha-D-glucose + (20S)-ginsenoside Rh2 | - |
Panax ginseng | UDP + (20S)-ginsenoside Rg3 | - |
? | |
2.4.1.365 | UDP-alpha-D-glucose + ginsenoside F2 | - |
Panax ginseng | UDP + ginsenoside Rd | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.364 | UGT74AE2 | - |
Panax ginseng |
2.4.1.365 | UGT94Q2 | - |
Panax ginseng |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.364 | 0.0000556 | - |
(20S)-protopanaxadiol | at pH 8.0 and 35°C | Panax ginseng | |
2.4.1.364 | 0.0001347 | - |
ginsenoside C-K | at pH 8.0 and 35°C | Panax ginseng | |
2.4.1.365 | 0.41 | - |
ginsenoside F2 | at pH 8.0 and 35°C | Panax ginseng | |
2.4.1.365 | 8.6 | - |
(20S)-ginsenoside Rh2 | at pH 8.0 and 35°C | Panax ginseng |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.1.364 | metabolism | key enzyme for the synthesis of ginsenosides | Panax ginseng |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.364 | 0.00224 | - |
(20S)-protopanaxadiol | at pH 8.0 and 35°C | Panax ginseng | |
2.4.1.364 | 0.00337 | - |
ginsenoside C-K | at pH 8.0 and 35°C | Panax ginseng | |
2.4.1.365 | 2.183 | - |
ginsenoside F2 | at pH 8.0 and 35°C | Panax ginseng | |
2.4.1.365 | 27.683 | - |
(20S)-ginsenoside Rh2 | at pH 8.0 and 35°C | Panax ginseng |