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Literature summary extracted from

  • Jung, S.; Kim, W.; Park, S.; Jeong, J.; Park, M.; Lim, S.; Lee, Y.; Im, W.; Lee, J.; Choi, G.; Kim, S.
    Two ginseng UDP-glycosyltransferases synthesize ginsenoside Rg 3 and Rd (2014), Plant Cell Physiol., 55, 2177-2188 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.364 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells and in Saccharomyces cerevisiae Panax ginseng
2.4.1.365 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells and in Saccharomyces cerevisiae Panax ginseng

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.364 0.025
-
(20S)-protopanaxadiol at pH 8.0 and 35°C Panax ginseng
2.4.1.364 0.04
-
ginsenoside C-K at pH 8.0 and 35°C Panax ginseng
2.4.1.365 0.188
-
ginsenoside F2 at pH 8.0 and 35°C Panax ginseng
2.4.1.365 0.311
-
(20S)-ginsenoside Rh2 at pH 8.0 and 35°C Panax ginseng

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.364 UDP-alpha-D-glucose + (20S)-protopanaxadiol Panax ginseng
-
UDP + (20S)-ginsenoside Rh2
-
?
2.4.1.364 UDP-alpha-D-glucose + ginsenoside C-K Panax ginseng
-
UDP + ginsenoside F2
-
?
2.4.1.365 UDP-alpha-D-glucose + (20S)-ginsenoside Rh2 Panax ginseng
-
UDP + (20S)-ginsenoside Rg3
-
?
2.4.1.365 UDP-alpha-D-glucose + ginsenoside F2 Panax ginseng
-
UDP + ginsenoside Rd
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.364 Panax ginseng A0A0A6ZFR4
-
-
2.4.1.365 Panax ginseng A0A0A6ZFY4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.364 glutathione-Sepharose column chromatography Panax ginseng
2.4.1.365 glutathione-Sepharose column chromatography Panax ginseng

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.364 leaf
-
Panax ginseng
-
2.4.1.364 root highest expression Panax ginseng
-
2.4.1.365 leaf
-
Panax ginseng
-
2.4.1.365 root highest expression Panax ginseng
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.364 additional information the enzyme glucosylates the C3 position of both (20S)-protopanaxadiol and ginsenoside C-K, but not C20 or C12 positions and also does not glucosylate hydroxyl residues at C3, C6, C12 or C20 positions in any protopanaxadiol-type ginsenoside Panax ginseng ?
-
?
2.4.1.364 UDP-alpha-D-glucose + (20S)-protopanaxadiol
-
Panax ginseng UDP + (20S)-ginsenoside Rh2
-
?
2.4.1.364 UDP-alpha-D-glucose + ginsenoside C-K
-
Panax ginseng UDP + ginsenoside F2
-
?
2.4.1.365 additional information the enzyme glucosylates the C3-O-glucoside of both (20S)-ginsenoside Rh2 and ginsenoside F2, but not the C20-O-glucoside of ginsenoside C-K and ginsenoside F2, also displaying strong substrate specificity and regioselectivity Panax ginseng ?
-
?
2.4.1.365 UDP-alpha-D-glucose + (20S)-ginsenoside Rh2
-
Panax ginseng UDP + (20S)-ginsenoside Rg3
-
?
2.4.1.365 UDP-alpha-D-glucose + ginsenoside F2
-
Panax ginseng UDP + ginsenoside Rd
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.364 UGT74AE2
-
Panax ginseng
2.4.1.365 UGT94Q2
-
Panax ginseng

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.364 0.0000556
-
(20S)-protopanaxadiol at pH 8.0 and 35°C Panax ginseng
2.4.1.364 0.0001347
-
ginsenoside C-K at pH 8.0 and 35°C Panax ginseng
2.4.1.365 0.41
-
ginsenoside F2 at pH 8.0 and 35°C Panax ginseng
2.4.1.365 8.6
-
(20S)-ginsenoside Rh2 at pH 8.0 and 35°C Panax ginseng

General Information

EC Number General Information Comment Organism
2.4.1.364 metabolism key enzyme for the synthesis of ginsenosides Panax ginseng

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.364 0.00224
-
(20S)-protopanaxadiol at pH 8.0 and 35°C Panax ginseng
2.4.1.364 0.00337
-
ginsenoside C-K at pH 8.0 and 35°C Panax ginseng
2.4.1.365 2.183
-
ginsenoside F2 at pH 8.0 and 35°C Panax ginseng
2.4.1.365 27.683
-
(20S)-ginsenoside Rh2 at pH 8.0 and 35°C Panax ginseng