Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mehra, P.; Pandey, B.K.; Verma, L.; Giri, J.
    A novel glycerophosphodiester phosphodiesterase improves phosphate deficiency tolerance in rice (2018), Plant Cell Environ., 42, 1167-1179 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.2 expressed in Escherichia coli BL21(DE3) cells Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.2 EDTA
-
 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.2 0.56
-
 sn-glycero-3-phosphocholine at pH 7.5 and 45°C Oryza sativa
3.1.4.2 1.06
-
 sn-glycero-3-phosphoethanolamine at pH 7.5 and 45°C Oryza sativa
3.1.4.2 7.25
-
 sn-glycero-3-phosphoinositol at pH 7.5 and 45°C Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.2 Co2+ the enzyme shows maximum activity at very low concentrations (less than 0.05 mM) of Co2+ Oryza sativa
3.1.4.2 Mg2+ preferred cofactor. The enzyme activity increases with increasing concentrations of Mg2+ (up to 3 mM) Oryza sativa
3.1.4.2 Mn2+ the enzyme shows maximum activity at very low concentrations (less than 0.05 mM) of Mn2+ Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.2 Oryza sativa
-
-
-
3.1.4.46 Oryza sativa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.2 seedling
-
 Oryza sativa
-
3.1.4.46 root
-
 Oryza sativa
-
3.1.4.46 shoot
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.2 0.016
-
 with sn-glycero-3-phosphoinositol as substrate, at pH 7.5 and 45°C Oryza sativa
3.1.4.2 0.108
-
 with sn-glycero-3-phosphocholine as substrate, at pH 7.5 and 45°C Oryza sativa
3.1.4.2 0.126
-
 with sn-glycero-3-phosphoethanolamine as substrate, at pH 7.5 and 45°C Oryza sativa
3.1.4.46 107.6
-
 substrate glycerophosphocholine, pH not specified in the publication, temperature not specified in the publication Oryza sativa
3.1.4.46 125.8
-
 substrate glycerophosphoethanolamine, pH not specified in the publication, temperature not specified in the publication Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.2 sn-glycero-3-phosphocholine + H2O
-
 Oryza sativa choline + sn-glycerol 3-phosphate
-
 ?
3.1.4.2 sn-glycero-3-phosphoethanolamine + H2O
-
 Oryza sativa sn-glycerol-3-phosphate + ethanolamine
-
 ?
3.1.4.2 sn-glycero-3-phosphoinositol + H2O
-
 Oryza sativa sn-glycerol-3-phosphate + inositol
-
 ?
3.1.4.46 glycerophosphocholine + H2O
-
 Oryza sativa choline + glycerol-3-phosphate
-
 ?
3.1.4.46 glycerophosphoethanolamine + H2O
-
 Oryza sativa ethanolamine + glycerol-3-phosphate
-
 ?
3.1.4.46 glycerophosphoinositol + H2O less than 10% of the activity with glycerophosphocholine Oryza sativa inositol + glycerol-3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.2 ? x * 423500, recombinant His6-tagged enzyme, SDS-PAGE Oryza sativa
3.1.4.46 ? x * 42350, SDS-PAGE, recombinant protein with His-tag Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
3.1.4.2 GDPD2
-
 Oryza sativa
3.1.4.2 glycerophosphodiester phosphodiesterase
-
 Oryza sativa
3.1.4.46 GDPD2
-
 Oryza sativa
3.1.4.46 Os02g31030
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.2 45
-
-
 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.2 7.5
-
 any rise in pH above 7.5 results in a decrease of enzyme activity Oryza sativa
3.1.4.46 7.5
-
-
 Oryza sativa

Expression

EC Number Organism Comment Expression
3.1.4.2 Oryza sativa the enzyme is transcriptionally induced by phosphate deficiency up
3.1.4.46 Oryza sativa expression in root and shoot is induced by phosphate deficiency and transcriptionally regulated by PHR2 up

General Information

EC Number General Information Comment Organism
3.1.4.2 physiological function the enzyme plays a role in low phosphate acclimation in rice Oryza sativa
3.1.4.46 physiological function overexpression lines show higher GDPD activity, phosphate content, root growth, and biomass accumulation as compared with wild type. Silencing of GDPD2 leads to decreased GDPD activity and phosphate content. Most of the phosphate-containing metabolites and fatty acids are elevated in transgenic lines and in overexpression lines higher accumulation of several classes of phospholipids and galactolipids is observed Oryza sativa