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Literature summary extracted from

  • Fliegmann, J.; Furtwaengler, K.; Malterer, G.; Cantarello, C.; Schueler, G.; Ebel, J.; Mithoefer, A.
    Flavone synthase II (CYP93B16) from soybean (Glycine max L.) (2010), Phytochemistry, 71, 508-514 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.76 expression in Saccharomyces cerevisiae Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.76 0.0018
-
eriodictyol pH 7.9, 25°C Glycine max
1.14.19.76 0.0025
-
naringenin pH 7.9, 25°C Glycine max
1.14.19.76 0.0042
-
liquiritigenin pH 7.9, 25°C Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.76 membrane bound to Glycine max 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.76 eriodictyol + [reduced NADPH-hemoprotein reductase] + O2 Glycine max
-
luteolin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?
1.14.19.76 liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 Glycine max
-
7,4'-dihydroxyflavone + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?
1.14.19.76 naringenin + [reduced NADPH-hemoprotein reductase] + O2 Glycine max
-
apigenin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.76 Glycine max D3U591
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.76 eriodictyol + [reduced NADPH-hemoprotein reductase] + O2
-
Glycine max luteolin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?
1.14.19.76 eriodictyol + [reduced NADPH-hemoprotein reductase] + O2 the enzymatic reaction of FNS II is stereoselective, favouring the (S)-enantiomer over the (R)-enantiomer Glycine max luteolin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?
1.14.19.76 liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2
-
Glycine max 7,4'-dihydroxyflavone + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?
1.14.19.76 liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 the enzymatic reaction of FNS II is stereoselective, favouring the (S)-enantiomer over the (R)-enantiomer Glycine max 7,4'-dihydroxyflavone + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?
1.14.19.76 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
Glycine max apigenin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?
1.14.19.76 naringenin + [reduced NADPH-hemoprotein reductase] + O2 the enzymatic reaction of FNS II is stereoselective, favouring the (S)-enantiomer over the (R)-enantiomer Glycine max apigenin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.76 CYP93B16
-
Glycine max
1.14.19.76 FNS II
-
Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.76 25
-
-
Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.19.76 7.9
-
-
Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.76 cytochrome P-450 heme-thiolate protein Glycine max

Expression

EC Number Organism Comment Expression
1.14.19.76 Glycine max induced by coronalon and by 12-oxo-phytodienoic acid. Jasmonic acid at a concentration of 0.1 mM is a comparatively weak inductor up

General Information

EC Number General Information Comment Organism
1.14.19.76 evolution phylogenetic analyses of the subfamily of plant CYP93B enzymes indicate the evolution of a gene encoding a flavone synthase which originally catalyzes the direct conversion of flavanones into flavones, via early gene duplication into a less efficient enzyme with an altered catalytic mechanism. Ultimately, this allows the evolution of the legume-specific isoflavonoid synthase activity Glycine max