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Literature summary extracted from

  • Khatoon, H.; Mansfeld, J.; Schierhorn, A.; Ulbrich-Hofmann, R.
    Purification, sequencing and characterization of phospholipase D from Indian mustard seeds (2015), Phytochemistry, 117, 65-75 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 phosphatidylinositol 4,5-bisphosphate strong activating effect at 2-10 mM CaCl2 Brassica juncea var. juncea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 Na3VO4
-
 Brassica juncea var. juncea
3.1.4.4 NaAlF4
-
 Brassica juncea var. juncea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.4 1.84
-
 phosphatidylcholine pH 5.5, 37°C Brassica juncea var. juncea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ maximum activity above 60 mM Brassica juncea var. juncea

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Brassica juncea var. juncea A0A0G4AKV1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 43.6
-
 substrate phosphatidylglycerol, pH 5.5, 37°C Brassica juncea var. juncea
3.1.4.4 117.8
-
 substrate phosphatidylethanolamine, pH 5.5, 37°C Brassica juncea var. juncea
3.1.4.4 253.8
-
 substrate phosphatidylcholine, pH 5.5, 37°C Brassica juncea var. juncea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 additional information phosphatidylcholines with short-chain fatty acids are better substrates than phosphatidylcholines with long fatty acid chains. Lysophosphatidylcholine is not accepted as substrate Brassica juncea var. juncea ?
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Brassica juncea var. juncea choline + phosphatidate
-
 ?
3.1.4.4 phosphatidylethanolamine + H2O
-
 Brassica juncea var. juncea ethanolamine + phosphatidate
-
 ?
3.1.4.4 phosphatidylglycerol + H2O
-
 Brassica juncea var. juncea glycerol + phosphatidate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.4 ? x * 92000, SDS-PAGE and calculated from sequence Brassica juncea var. juncea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.4 45
-
 inactivation above Brassica juncea var. juncea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 5.5 6
-
 Brassica juncea var. juncea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.4.4 Brassica juncea var. juncea calculated from sequence
-
 5.4

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.4.4 0.0078
-
 pH 5.5, 37°C Brassica juncea var. juncea NaAlF4
3.1.4.4 0.033
-
 pH 5.5, 37°C Brassica juncea var. juncea Na3VO4