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Literature summary extracted from

  • Campe, R.; Hollenbach, E.; Kaemmerer, L.; Hendriks, J.; Hoeffken, H.W.; Kraus, H.; Lerchl, J.; Mietzner, T.; Tresch, S.; Witschel, M.; Hutzler, J.
    A new herbicidal site of action Cinmethylin binds to acyl-ACP thioesterase and inhibits plant fatty acid biosynthesis (2018), Pestic. Biochem. Physiol., 148, 116-125 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.14 expressed in Escherichia coli BL21 DE3 Star cells Lemna

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.2.14 in complex with cinmethylin, sitting drop vapor diffusion method, using 20% (w/v) PEG 5K and 0.1M BIS-TRIS at pH 6.5 Lemna

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.14 cinmethylin
-
Lemna

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.14 plastid
-
Lemna 9536
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.14 an oleoyl-[acyl-carrier protein] + H2O Lemna
-
an [acyl-carrier protein] + oleate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.14 Lemna
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.14 Ni-IDA column chromatography Lemna

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.14 an oleoyl-[acyl-carrier protein] + H2O
-
Lemna an [acyl-carrier protein] + oleate
-
?
3.1.2.14 linolenoyl-[acyl-carrier protein] + H2O
-
Lemna [acyl-carrier protein] + linolenate
-
?
3.1.2.14 linoleoyl-[acyl-carrier protein] + H2O
-
Lemna [acyl-carrier protein] + linoleate
-
?
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O preferred substrate Lemna [acyl-carrier protein] + oleate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.14 acyl-ACP-thioesterase
-
Lemna
3.1.2.14 FAT A
-
Lemna
3.1.2.14 fatty acid thioesterase
-
Lemna