Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, P.; Xu, R.; Wang, J.; Wu, Z.; Yan, L.; Zhao, W.; Liu, Y.; Ma, W.; Shi, X.; Li, H.
    Starch biotransformation into isomaltooligosaccharides using thermostable alpha-glucosidase from Geobacillus stearothermophilus (2018), PeerJ, 6, e5086 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.20 synthesis the alpha-transglucosidase-producing Geobacillus stearothermophilus as a potential application technique can be successfully used to prepare industrial isomaltooligosaccharides (IMOs) Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.20 additional information biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2. 5 l and 20 l scale fermentations are performed. After isolation and purification, liquid alpha-glucosidase preparations are obtained. Through covalent cross-linking and adsorption cross-linking using magnetic chitosan microspheres as the carrier and glutaraldehyde as the crosslinking agent, the conditions for immobilization of alpha-glucosidase on chitosan are determined. Isomaltooligosaccharides (IMOs) are prepared using chitosan membrane-immobilized alpha-glucosidase, beta-amylase, pullulanase, fungal alpha-amylase and starch as substrate. The mixed syrup that contains IMOs is evaluated and analyzed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). In addition, small-scale preparation of IMOs is performed. Method, overview Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 Co2+ slight inhibition Geobacillus stearothermophilus
3.2.1.20 Cu2+ strong inhibition Geobacillus stearothermophilus
3.2.1.20 Fe2+ moderate inhibition Geobacillus stearothermophilus
3.2.1.20 Zn2+ moderate inhibition Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.20 Ca2+ activates Geobacillus stearothermophilus
3.2.1.20 K+ activates Geobacillus stearothermophilus
3.2.1.20 Mg2+ activates Geobacillus stearothermophilus
3.2.1.20 Mn2+ activates Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.20 maltose + H2O Geobacillus stearothermophilus
-
 alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltose + H2O Geobacillus stearothermophilus U2
-
 alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 additional information Geobacillus stearothermophilus biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2 ?
-
 ?
3.2.1.20 additional information Geobacillus stearothermophilus U2 biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2 ?
-
 ?
3.2.1.20 starch + H2O Geobacillus stearothermophilus
-
 ?
-
 ?
3.2.1.20 starch + H2O Geobacillus stearothermophilus
-
 alpha-D-glucose + ?
-
 ?
3.2.1.20 starch + H2O Geobacillus stearothermophilus U2
-
 alpha-D-glucose + ?
-
 ?
3.2.1.20 starch + H2O Geobacillus stearothermophilus U2
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Geobacillus stearothermophilus
-
-
-
3.2.1.20 Geobacillus stearothermophilus Q9R5L9 i.e. Bacillus stearothermophilus
-
3.2.1.20 Geobacillus stearothermophilus U2
-
-
-
3.2.1.20 Geobacillus stearothermophilus U2 Q9R5L9 i.e. Bacillus stearothermophilus
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 additional information optimization of culture conditions for wild-type and engineered strains, overview Geobacillus stearothermophilus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Geobacillus stearothermophilus 4-nitrophenol + D-glucose
-
 ?
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Geobacillus stearothermophilus U2 4-nitrophenol + D-glucose
-
 ?
3.2.1.20 4-nitrophenyl alpha-D-glucoside + H2O
-
 Geobacillus stearothermophilus 4-nitrophenol + alpha-D-glucose
-
 ?
3.2.1.20 4-nitrophenyl alpha-D-glucoside + H2O
-
 Geobacillus stearothermophilus U2 4-nitrophenol + alpha-D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Geobacillus stearothermophilus alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Geobacillus stearothermophilus U2 alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 additional information biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2 Geobacillus stearothermophilus ?
-
 ?
3.2.1.20 additional information biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2 Geobacillus stearothermophilus U2 ?
-
 ?
3.2.1.20 starch + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
3.2.1.20 starch + H2O
-
 Geobacillus stearothermophilus U2 ?
-
 ?
3.2.1.20 starch + H2O
-
 Geobacillus stearothermophilus alpha-D-glucose + ?
-
 ?
3.2.1.20 starch + H2O
-
 Geobacillus stearothermophilus U2 alpha-D-glucose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 alpha-transglucosidase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 45
-
 free enzyme Geobacillus stearothermophilus
3.2.1.20 50
-
 enzyme immobilized on magnetic chitosan microspheres Geobacillus stearothermophilus
3.2.1.20 60
-
-
 Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.20 35 55 the free enzyme is active between 35 and 55°C Geobacillus stearothermophilus
3.2.1.20 40 60 the enzyme immobilized on magnetic chitosan microspheres shows about 40% activity at 30°C, about 50% activity at 40°C, 100% activity at 50°C, and about 90% activity at 60°C Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 20 80 the enzyme is thermostable for 24 h and no loss in activity is found below 37°C, but it is inactivated at 65°C or more and completely loses its activity at 80°C Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 6.8
-
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 6.2 7.4 the enzyme is active between pH 6.2 and 7.4 Geobacillus stearothermophilus