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Literature summary extracted from

  • Fang, Y.; Zhang, L.; Wang, J.; Zhou, Y.; Ye, B.
    Biodegradation of phthalate esters by a newly isolated Acinetobacter sp. strain LMB-5 and characteristics of its esterase (2017), Pedosphere, 27, 606-615 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.60 expression in Escherichia coli Acinetobacter sp. LMB-5

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.60 Ca2+ 1 mM, 45.5% residual activity Acinetobacter sp. LMB-5
3.1.1.60 K+ 1 mM, 55.8% residual activity Acinetobacter sp. LMB-5
3.1.1.60 Mg2+ 1 mM, 15.4% residual activity Acinetobacter sp. LMB-5
3.1.1.60 Na+ 1 mM, 54.3% residual activity Acinetobacter sp. LMB-5
3.1.1.60 Zn2+ 1 mM, 21.5% residual activity Acinetobacter sp. LMB-5

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.60 Cu2+ 1 mM, 127% of initial activity Acinetobacter sp. LMB-5
3.1.1.60 Fe3+ 1 mM, 162% of initial activity Acinetobacter sp. LMB-5

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.60 dimethyl sulfoxide high activity in the presence of 10% v/v Acinetobacter sp. LMB-5
3.1.1.60 Methanol high activity in the presence of 10% v/v Acinetobacter sp. LMB-5

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.60 Acinetobacter sp. LMB-5
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.60 bis(2-ethylhexyl)phthalate + H2O
-
 Acinetobacter sp. LMB-5 2-ethylhexyl phthalate + 2-ethylhexan-1-ol
-
 ?
3.1.1.60 dibutylphthalate + H2O
-
 Acinetobacter sp. LMB-5 butylphthalate + butanol
-
 ?
3.1.1.60 diethylphthalate + H2O
-
 Acinetobacter sp. LMB-5 ethylphthalate + ethanol
-
 ?
3.1.1.60 dimethylphthalate + H2O
-
 Acinetobacter sp. LMB-5 methylphthalate + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.60 ? x * 40340, calculated from sequence, x * 40000, SDS-PAGE Acinetobacter sp. LMB-5

Synonyms

EC Number Synonyms Comment Organism
3.1.1.60 Est 3563
-
 Acinetobacter sp. LMB-5

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.60 40
-
 rapid loss of activity above Acinetobacter sp. LMB-5

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.60 7
-
-
 Acinetobacter sp. LMB-5

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.60 5 7
-
 Acinetobacter sp. LMB-5

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.60 Acinetobacter sp. LMB-5 calculated from sequence
-
 9.1

General Information

EC Number General Information Comment Organism
3.1.1.60 physiological function strains grows in mineral salt medium containing dimethyl phthalate, diethyl phthalate, di-n-butyl phthalate, and di-(2-ethylhexyl) phthalate. The consumption of dimethyl phthalate, diethyl phthalate, di-n-butyl phthalate is up to 98.87%, 94.94%, and 72.15%, respectively Acinetobacter sp. LMB-5