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Literature summary extracted from

  • Habib-ur-Rehma, H.; Siddiqui, M.; Qayyum, A.; Bano, A.; Rashid, N.
    Gene expression in Escherichia coli and purification of recombinant type II pullulanase from a hyperthermophilic archaeon, Pyrobaculum calidifontis (2018), Pak. J. Zool., 50, 1381-1386 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 expression in Escherichia coli Pyrobaculum calidifontis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 additional information Ca2+-independent enzyme Pyrobaculum calidifontis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Pyrobaculum calidifontis A3MWL6
-
-
3.2.1.41 Pyrobaculum calidifontis VA1 A3MWL6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41
-
 Pyrobaculum calidifontis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
 Pyrobaculum calidifontis maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose
-
 ?
3.2.1.41 amylopectin + H2O
-
 Pyrobaculum calidifontis VA1 maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose
-
 ?
3.2.1.41 pullulan + H2O
-
 Pyrobaculum calidifontis maltotriose + maltose
-
 ?
3.2.1.41 pullulan + H2O
-
 Pyrobaculum calidifontis VA1 maltotriose + maltose
-
 ?
3.2.1.41 starch + H2O
-
 Pyrobaculum calidifontis maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose
-
 ?
3.2.1.41 starch + H2O
-
 Pyrobaculum calidifontis VA1 maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 Pcal-1616
-
 Pyrobaculum calidifontis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 95
-
-
 Pyrobaculum calidifontis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.41 60 110 60°C: about 60% of maximal activity, 110°C: about 90% of maximal activity Pyrobaculum calidifontis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 5.5
-
-
 Pyrobaculum calidifontis