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Literature summary extracted from

  • Ahmed Al-Tammar, K.; Omar, O.; Abdul Murad, A.; Abu Bakar, F.
    Expression and characterization of a cutinase (AnCUT2) from Aspergillus niger (2016), Open Life Sci., 11, 29-38 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.74 degradation enzyme is able to modify the surface of the polycaprolactone and polyethylene terephthalate synthetic polyesters Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.74 expression in Pichia pastoris Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.74 additional information not inhibitory: dithiothreitol, EDTA, urea Aspergillus niger
3.1.1.74 PMSF 1 mM, less than 20% residual activity Aspergillus niger
3.1.1.74 SDS 1 mM, less than 5% residual activity Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.74 Aspergillus niger A0A0F6TMI2 isoform Cut2
-
3.1.1.74 Aspergillus niger ATCC 10574 A0A0F6TMI2 isoform Cut2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.74 4-nitrophenyl butanoate + H2O
-
 Aspergillus niger 4-nitrophenol + butanoate
-
 ?
3.1.1.74 4-nitrophenyl butanoate + H2O
-
 Aspergillus niger ATCC 10574 4-nitrophenol + butanoate
-
 ?
3.1.1.74 poly(caprolactone) + H2O
-
 Aspergillus niger ?
-
 ?
3.1.1.74 poly(caprolactone) + H2O
-
 Aspergillus niger ATCC 10574 ?
-
 ?
3.1.1.74 polyethylene terephthalate + H2O
-
 Aspergillus niger ?
-
 ?
3.1.1.74 polyethylene terephthalate + H2O
-
 Aspergillus niger ATCC 10574 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.74 ? x * 2767, calculated from sequence, x * 40000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.74 40
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.74 40
-
 1 h, more than 80% residual activity Aspergillus niger
3.1.1.74 50
-
 1 h, 40% residual activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.74 5 6
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.74 2 10 1 h, more than 60% residual activity Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.74 Aspergillus niger calculated from sequence
-
 4.7