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Literature summary extracted from

  • Chembazhi, U.V.; Patil, V.V.; Sah, S.; Reeve, W.; Tiwari, R.P.; Woo, E.; Varshney, U.
    Uracil DNA glycosylase (UDG) activities in Bradyrhizobium diazoefficiens characterization of a new class of UDG with broad substrate specificity (2017), Nucleic Acids Res., 45, 5863-5876 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.27 expressed in Escherichia coli strain CC102 Bradyrhizobium diazoefficiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.27 A59Y the mutation leads to a decrease in its activity on xanthine, and 5-hydroxymethyluracil containing single stranded DNAs but not on uracil containing single stranded DNA Bradyrhizobium diazoefficiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.27 additional information not inhibited by ctcaagtg-tetrahydrofuran-aggcatgcaagagct, DNA with abasic site, and Ugi protein Bradyrhizobium diazoefficiens
3.2.2.27 Uracil free uracil causes a 5fold reduction in enzyme activity at 10 mM concentration Bradyrhizobium diazoefficiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.27 uracil-containing single-stranded DNA + H2O Bradyrhizobium diazoefficiens
-
 uracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-containing single-stranded DNA + H2O Bradyrhizobium diazoefficiens USDA110
-
 uracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O Bradyrhizobium diazoefficiens
-
 uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O Bradyrhizobium diazoefficiens USDA110
-
 uracil + double-stranded DNA with abasic site
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.27 Bradyrhizobium diazoefficiens Q89XR0 previously Bradyrhizobium japonicum
-
3.2.2.27 Bradyrhizobium diazoefficiens USDA110 Q89XR0 previously Bradyrhizobium japonicum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.27 Ni-NTA column chromatography Bradyrhizobium diazoefficiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.27 hydroxymethyluracil-containing single-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens 5-hydroxymethyluracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 hydroxymethyluracil-containing single-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens USDA110 5-hydroxymethyluracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 additional information the enzyme does not utilize DNA oligomer containing inosine or thymine in single stranded DNA or double stranded DNA Bradyrhizobium diazoefficiens ?
-
 ?
3.2.2.27 additional information the enzyme prefers single stranded DNA Bradyrhizobium diazoefficiens ?
-
 ?
3.2.2.27 additional information the enzyme does not utilize DNA oligomer containing inosine or thymine in single stranded DNA or double stranded DNA Bradyrhizobium diazoefficiens USDA110 ?
-
 ?
3.2.2.27 additional information the enzyme prefers single stranded DNA Bradyrhizobium diazoefficiens USDA110 ?
-
 ?
3.2.2.27 uracil-containing single-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens uracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-containing single-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens USDA110 uracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-guanine-mismatched double-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens USDA110 uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 xanthine-containing single-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens xanthine + single-stranded DNA with abasic site
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.27 Blr0248
-
 Bradyrhizobium diazoefficiens
3.2.2.27 UDG
-
 Bradyrhizobium diazoefficiens
3.2.2.27 UNG
-
 Bradyrhizobium diazoefficiens
3.2.2.27 uracil DNA glycosylase
-
 Bradyrhizobium diazoefficiens