EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.26.3 | gene RNT1, recombinant expression | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.26.3 | additional information | product inhibition, presence of 800 nM products reduces the value of the initial phase kcat by 5fold. The reduction of Rnt1p activity by the product is impaired when the reaction is carried out with a buffer containing 500 mM KCl, which reduces the stability of Rnt1p/RNA complex. Addition of Xrn1p, which degrades the reaction products, to the cleavage reaction significantly increases the steady-state catalytic rate of Rnt1p without affecting the initial burst phase | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.26.3 | additional information | - |
additional information | real-time analysis of Rnt1p cleavage kinetics, RNA binding to purified Rnt1p is monitored, Michaelis-Menten kinetics | Saccharomyces cerevisiae | |
3.1.26.3 | 0.11 | - |
RNA | pH 8.5, 30°C, recombinant enzyme | Saccharomyces cerevisiae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.26.3 | KCl | KCl reduces the stability of Rnt1p/RNA complex | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.26.3 | Saccharomyces cerevisiae | C7GRB2 | - |
- |
3.1.26.3 | Saccharomyces cerevisiae JAY291 | C7GRB2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.26.3 | recombinant enzyme | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.3 | additional information | the catalytic efficiency of yeast ribonuclease III depends on substrate specific product release rate. Development of a real-time FRET assay for the detection of dsRNA degradation by yeast RNase III (Rnt1p) and detection of kinetic bottlenecks controlling the reactivity of different substrates. Rnt1p cleavage reaction is not only limited by the rate of catalysis but can also depend on base-pairing of product termini. Cleavage products terminating with paired nucleotides, like the degradation signals found in coding mRNA sequence, were less reactive and more prone to inhibition than products having unpaired nucleotides found in noncoding RNA substrates | Saccharomyces cerevisiae | ? | - |
? | |
3.1.26.3 | additional information | the catalytic efficiency of yeast ribonuclease III depends on substrate specific product release rate. Development of a real-time FRET assay for the detection of dsRNA degradation by yeast RNase III (Rnt1p) and detection of kinetic bottlenecks controlling the reactivity of different substrates. Rnt1p cleavage reaction is not only limited by the rate of catalysis but can also depend on base-pairing of product termini. Cleavage products terminating with paired nucleotides, like the degradation signals found in coding mRNA sequence, were less reactive and more prone to inhibition than products having unpaired nucleotides found in noncoding RNA substrates | Saccharomyces cerevisiae JAY291 | ? | - |
? | |
3.1.26.3 | RNA + H2O | RNA substrate tested are U5, U2, Mig2, and Yta6. Comparison between the association and dissociation kinetics of Mig2 and U5 products indicated that Mig2 products have a 2fold higher association rate and an 8fold lower dissociation rate. The reactivity of Rnt1p substrates is defined by the basepairing of the cleavage site, substrate specificity, overview | Saccharomyces cerevisiae | ? | - |
? | |
3.1.26.3 | RNA + H2O | RNA substrate tested are U5, U2, Mig2, and Yta6. Comparison between the association and dissociation kinetics of Mig2 and U5 products indicated that Mig2 products have a 2fold higher association rate and an 8fold lower dissociation rate. The reactivity of Rnt1p substrates is defined by the basepairing of the cleavage site, substrate specificity, overview | Saccharomyces cerevisiae JAY291 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.26.3 | RNase III | - |
Saccharomyces cerevisiae |
3.1.26.3 | RNT1 | - |
Saccharomyces cerevisiae |
3.1.26.3 | Rnt1p | - |
Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.26.3 | 30 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.26.3 | 1.08 | - |
RNA | pH 8.5, 30°C, recombinant enzyme | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.26.3 | 8.5 | - |
assay at | Saccharomyces cerevisiae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.26.3 | evolution | the enzyme is a member of the ribonuclease III (RNase III) family | Saccharomyces cerevisiae |
3.1.26.3 | physiological function | members of the ribonuclease III (RNase III) family regulate gene expression by triggering the degradation of double stranded RNA (dsRNA) | Saccharomyces cerevisiae |