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Literature summary extracted from

  • Evangelista, D.E.; de Araujo, E.A.; Neto, M.O.; Kadowaki, M.A.S.; Polikarpov, I.
    Biochemical characterization and low-resolution SAXS structure of an exo-polygalacturonase from Bacillus licheniformis (2018), New Biotechnol., 40, 268-274 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.67 expressed in Escherichia coli Rosetta cells Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 2-mercaptoethanol 89.7% residual activity at 4 mM Bacillus licheniformis
3.2.1.67 Ca2+ 74.7% residual activity at 4 mM Bacillus licheniformis
3.2.1.67 Co2+ 36% residual activity at 4 mM Bacillus licheniformis
3.2.1.67 Cu2+ 30.4% residual activity at 4 mM Bacillus licheniformis
3.2.1.67 dithiothreitol 83.3% residual activity at 4 mM Bacillus licheniformis
3.2.1.67 Fe2+ 11.76% residual activity at 4 mM Bacillus licheniformis
3.2.1.67 Fe3+ 64.6% residual activity at 4 mM Bacillus licheniformis
3.2.1.67 K+ 96.2% residual activity at 4 mM Bacillus licheniformis
3.2.1.67 Li+ 88% residual activity at 4 mM Bacillus licheniformis
3.2.1.67 Mg2+ 91.9% residual activity at 4 mM Bacillus licheniformis
3.2.1.67 Mn2+ 68.5% residual activity at 4 mM Bacillus licheniformis
3.2.1.67 Ni2+ 73.5% residual activity at 4 mM Bacillus licheniformis
3.2.1.67 SDS 24.2% residual activity at 0.1% (w/v) Bacillus licheniformis
3.2.1.67 Triton X-100 96.6% residual activity at 0.1% (v/v) Bacillus licheniformis
3.2.1.67 Tween 20 87.4% residual activity at 0.1% (v/v) Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Bacillus licheniformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 nickel affinity column chromatography and Superdex 200 gel filtration Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 apple pectin + H2O 2% activity compared to polygalacturonic acid Bacillus licheniformis galacturonic acid
-
 ?
3.2.1.67 methylated pectin + H2O 20% activity compared to polygalacturonic acid Bacillus licheniformis galacturonic acid
-
 ?
3.2.1.67 additional information no activity with carboxymethyl cellulose and xylan Bacillus licheniformis ?
-
 ?
3.2.1.67 polygalacturonic acid + H2O 100% activity Bacillus licheniformis galacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 monomer 1 * 50000, SDS-PAGE Bacillus licheniformis
3.2.1.67 monomer 1 * 48140, calculated from amino acid sequence Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 exo-polygalacturonanase
-
 Bacillus licheniformis
3.2.1.67 exoPG
-
 Bacillus licheniformis
3.2.1.67 pectinase
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 60
-
-
 Bacillus licheniformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.67 50 70 the enzyme maintains 65% of the maximum activity at 50°C and about 30% at 70°C Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 50
-
 the enzyme maintains residual activity of greater than 80% at 50°C after 15 h of incubation and after 24 h its activity is over 60% of its maximum activity Bacillus licheniformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.67 2.58
-
 polygalacturonic acid at pH 6.5 and 60°C Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 6.5
-
-
 Bacillus licheniformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.67 5 10 after incubation of the enzyme for 30 min at pH ranging from 5.0 to 10.0, no significant loss of the original enzyme activity is observed Bacillus licheniformis