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Literature summary extracted from
- Insights into activity and inhibition from the crystal structure of human O-GlcNAcase (2017), Nat. Chem. Biol., 13, 613-615 .
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.2.1.169 | enzyme in apo and inhibited states, hanging drop vapor diffusion method | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.169 | PUGNAc | i.e. O-(2-acetamido-2-deoxy-D-glucopyranosylidenamino) N-phenylcarbamate | Homo sapiens |  |
| 3.2.1.169 | thiamet G | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.169 | 0.04 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.07 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.17 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.81 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 2.1 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 4 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.169 | 103000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.169 | [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O | Homo sapiens | - |
[protein]-L-serine + N-acetyl-D-glucosamine | - |
? | |
| 3.2.1.169 | [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O | Homo sapiens | - |
[protein]-L-threonine + N-acetyl-D-glucosamine | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.169 | Homo sapiens | O60502 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.169 | Ni-Sepharose column chromatography | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.169 | 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| 3.2.1.169 | [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O | - |
Homo sapiens | [protein]-L-serine + N-acetyl-D-glucosamine | - |
? | |
| 3.2.1.169 | [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O | - |
Homo sapiens | [protein]-L-threonine + N-acetyl-D-glucosamine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.169 | dimer | x-ray crystallography | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.169 | O-GlcNAc hydrolase | - |
Homo sapiens |
| 3.2.1.169 | OGA | - |
Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.169 | 0.08 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.1 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.13 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.2 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 1.5 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 1.8 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.169 | 0.00000008 | - |
thiamet G | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.00000025 | - |
thiamet G | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.00000043 | - |
thiamet G | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.0000058 | - |
thiamet G | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.0000066 | - |
PUGNAc | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.0000123 | - |
PUGNAc | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.00004 | - |
PUGNAc | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.00059 | - |
PUGNAc | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.0031 | - |
thiamet G | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.009 | - |
thiamet G | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.011 | - |
PUGNAc | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.06 | - |
PUGNAc | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.169 | 0.03 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.04 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 0.12 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 2.9 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 11 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.169 | 37 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
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