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Literature summary extracted from

  • Zhou, Y.; Chen, X.; Li, X.; Han, Y.; Wang, Y.; Yao, R.; Li, S.
    Purification and characterization of a new cold-adapted and thermo-tolerant chitosanase from marine bacterium Pseudoalteromonas sp. SY39 (2019), Molecules, 24, 183.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 AlCl3 1 mM, 91% loss of activity Pseudoalteromonas sp. SY39
3.2.1.132 CoCl2 1 mM, 28% loss of activity Pseudoalteromonas sp. SY39
3.2.1.132 CuSO4 1 mM, 86% loss of activity Pseudoalteromonas sp. SY39
3.2.1.132 EDTA 1 mM, 35% loss of activity Pseudoalteromonas sp. SY39
3.2.1.132 FeCl3 1 mM, 90% loss of activity Pseudoalteromonas sp. SY39
3.2.1.132 FeSO4 1 mM, 30% loss of activity Pseudoalteromonas sp. SY39
3.2.1.132 additional information ethanol, isopropanol and dimethyl sulfoxide (5 mM -100 mM) have no effect in activity Pseudoalteromonas sp. SY39
3.2.1.132 NiCl2 1 mM, 26% loss of activity Pseudoalteromonas sp. SY39
3.2.1.132 SDS 1 mM, 46% loss of activity Pseudoalteromonas sp. SY39
3.2.1.132 ZnCl2 1 mM, 64% loss of activity Pseudoalteromonas sp. SY39

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.132 CaCl2 1 mM, 1.2fold activation Pseudoalteromonas sp. SY39
3.2.1.132 KCl 1 mM, 1.2fold activation Pseudoalteromonas sp. SY39
3.2.1.132 MgSO4 1 mM, 1.1fold activation Pseudoalteromonas sp. SY39
3.2.1.132 NaCl addition of 0.001-0.5M NaCl increases the CsnM activity to 128% to 151% Pseudoalteromonas sp. SY39

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 28000
-
 SDS-PAGE Pseudoalteromonas sp. SY39

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Pseudoalteromonas sp. SY39
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Pseudoalteromonas sp. SY39

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O chitodisaccharide is the main product (69.9% of the total product) Pseudoalteromonas sp. SY39 chitobiose + chitooligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 28000, SDS-PAGE Pseudoalteromonas sp. SY39

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 CsnM
-
 Pseudoalteromonas sp. SY39

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 40
-
-
 Pseudoalteromonas sp. SY39

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.132 15 50 15°C: 49% of maximal activity, 45°C: 96% of maximal activity, 50°C: about 45% of maximal activity Pseudoalteromonas sp. SY39

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 30
-
 1 h, enzyme retains 25.4% of its initial activity Pseudoalteromonas sp. SY39
3.2.1.132 40
-
 1 h, enzyme retains 15.8% of its initial activity Pseudoalteromonas sp. SY39
3.2.1.132 100
-
 the enzyme recovers 95.2%, 89.1% and 88.1% of its initial activity after boiling for 5, 10 and 20 min, respectively Pseudoalteromonas sp. SY39

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5.9
-
 sodium acetate buffer Pseudoalteromonas sp. SY39

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 5 6.3 pH 5.0: about 70% of maximal activity, pH 6.3: about 60% of maximal activity Pseudoalteromonas sp. SY39

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 5.8 7.9 the enzyme retains over 80% of its original activity after incubation at pH ranging from 5.83 to 7.92 at 4°C for 24 h Pseudoalteromonas sp. SY39