Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cestari, I.; McLeland-Wieser, H.; Stuart, K.
    Nuclear phosphatidylinositol 5-phosphatase is essential for allelic exclusion of variant surface glycoprotein genes in trypanosomes (2019), Mol. Cell. Biol., 39, e00395-18 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.36 D360A/N362A significant decrease in catalytic activity Trypanosoma brucei
3.1.3.86 D360A/N362A the mutations completely eliminate enzyme activity toward 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate and significantly decrease the dephosphorylation activity of the enzyme for 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.36 0.0316
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate mutant D360A/N362A, pH not specified in the publication, temperature not specified in the publication Trypanosoma brucei
3.1.3.36 0.0738
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate wild-type, pH not specified in the publication, temperature not specified in the publication Trypanosoma brucei
3.1.3.36 0.088
-
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate wild-type, pH not specified in the publication, temperature not specified in the publication Trypanosoma brucei
3.1.3.86 0.0316
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate mutant enzyme D360A/N362A, at pH 7.5 and 25°C Trypanosoma brucei
3.1.3.86 0.0738
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma brucei
3.1.3.86 0.088
-
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.36 nucleus
-
Trypanosoma brucei 5634
-
3.1.3.86 nucleus
-
Trypanosoma brucei 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.86 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O Trypanosoma brucei
-
1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
?
3.1.3.86 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O Trypanosoma brucei
-
1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.36 Trypanosoma brucei
-
-
-
3.1.3.86 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.86 protein A Mag Sepharose column chromatography Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.36 1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate + H2O
-
Trypanosoma brucei 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
?
3.1.3.36 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
Trypanosoma brucei 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
-
?
3.1.3.86 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O
-
Trypanosoma brucei 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
?
3.1.3.86 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
Trypanosoma brucei 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.86 ? x * 75000, SDS-PAGE Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
3.1.3.36 tb927.11.6270
-
Trypanosoma brucei
3.1.3.86 phosphatidylinositol 5-phosphatase
-
Trypanosoma brucei
3.1.3.86 PIP5Pase
-
Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.36 28.2
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate mutant D360A/N362A, pH not specified in the publication, temperature not specified in the publication Trypanosoma brucei
3.1.3.36 57.5
-
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate wild-type, pH not specified in the publication, temperature not specified in the publication Trypanosoma brucei
3.1.3.36 140.5
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate wild-type, pH not specified in the publication, temperature not specified in the publication Trypanosoma brucei
3.1.3.86 28.2
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate mutant enzyme D360A/N362A, at pH 7.5 and 25°C Trypanosoma brucei
3.1.3.86 57.5
-
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma brucei
3.1.3.86 140.5
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma brucei

General Information

EC Number General Information Comment Organism
3.1.3.36 physiological function nuclear phosphatidylinositol 5-phosphatase (PIP5Pase) interacts with repressor activator protein RAP1 in a multiprotein complex and functions in the control of variant surface glycoprotein allelic exclusion. RAP1 binds PIP5Pase substrate phosphatidylinositol 3,4,5-trisphosphate and catalytic mutation of PIP5Pase results in simultaneous transcription of variant surface glycoproteins from all telomeric expression sites and from silent subtelomeric variant surface glycoprotein arrays. PIP5Pase and RAP1 bind to telomeric expression sites, especially at 70-bp repeats and telomeres, and their binding is altered by PIP5Pase inactivation or knockdown Trypanosoma brucei
3.1.3.86 physiological function the enzyme's activity controls 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate binding by RAP1 and, thus, transcriptional silencing of variant surface glycoprotein genes Trypanosoma brucei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.36 653
-
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate wild-type, pH not specified in the publication, temperature not specified in the publication Trypanosoma brucei
3.1.3.36 892
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate mutant D360A/N362A, pH not specified in the publication, temperature not specified in the publication Trypanosoma brucei
3.1.3.36 1900
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate wild-type, pH not specified in the publication, temperature not specified in the publication Trypanosoma brucei
3.1.3.86 653
-
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma brucei
3.1.3.86 892
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate mutant enzyme D360A/N362A, at pH 7.5 and 25°C Trypanosoma brucei
3.1.3.86 1900
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma brucei