EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.36 | D360A/N362A | significant decrease in catalytic activity | Trypanosoma brucei |
3.1.3.86 | D360A/N362A | the mutations completely eliminate enzyme activity toward 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate and significantly decrease the dephosphorylation activity of the enzyme for 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Trypanosoma brucei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.36 | 0.0316 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | mutant D360A/N362A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
3.1.3.36 | 0.0738 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
3.1.3.36 | 0.088 | - |
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
3.1.3.86 | 0.0316 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | mutant enzyme D360A/N362A, at pH 7.5 and 25°C | Trypanosoma brucei | |
3.1.3.86 | 0.0738 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | wild type enzyme, at pH 7.5 and 25°C | Trypanosoma brucei | |
3.1.3.86 | 0.088 | - |
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate | wild type enzyme, at pH 7.5 and 25°C | Trypanosoma brucei |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.36 | nucleus | - |
Trypanosoma brucei | 5634 | - |
3.1.3.86 | nucleus | - |
Trypanosoma brucei | 5634 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.86 | 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O | Trypanosoma brucei | - |
1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate | - |
? | |
3.1.3.86 | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | Trypanosoma brucei | - |
1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.36 | Trypanosoma brucei | - |
- |
- |
3.1.3.86 | Trypanosoma brucei | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.86 | protein A Mag Sepharose column chromatography | Trypanosoma brucei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.36 | 1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate + H2O | - |
Trypanosoma brucei | 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate | - |
? | |
3.1.3.36 | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Trypanosoma brucei | 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate | - |
? | |
3.1.3.86 | 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O | - |
Trypanosoma brucei | 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate | - |
? | |
3.1.3.86 | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Trypanosoma brucei | 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.86 | ? | x * 75000, SDS-PAGE | Trypanosoma brucei |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.36 | tb927.11.6270 | - |
Trypanosoma brucei |
3.1.3.86 | phosphatidylinositol 5-phosphatase | - |
Trypanosoma brucei |
3.1.3.86 | PIP5Pase | - |
Trypanosoma brucei |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.36 | 28.2 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | mutant D360A/N362A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
3.1.3.36 | 57.5 | - |
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
3.1.3.36 | 140.5 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
3.1.3.86 | 28.2 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | mutant enzyme D360A/N362A, at pH 7.5 and 25°C | Trypanosoma brucei | |
3.1.3.86 | 57.5 | - |
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate | wild type enzyme, at pH 7.5 and 25°C | Trypanosoma brucei | |
3.1.3.86 | 140.5 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | wild type enzyme, at pH 7.5 and 25°C | Trypanosoma brucei |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.3.36 | physiological function | nuclear phosphatidylinositol 5-phosphatase (PIP5Pase) interacts with repressor activator protein RAP1 in a multiprotein complex and functions in the control of variant surface glycoprotein allelic exclusion. RAP1 binds PIP5Pase substrate phosphatidylinositol 3,4,5-trisphosphate and catalytic mutation of PIP5Pase results in simultaneous transcription of variant surface glycoproteins from all telomeric expression sites and from silent subtelomeric variant surface glycoprotein arrays. PIP5Pase and RAP1 bind to telomeric expression sites, especially at 70-bp repeats and telomeres, and their binding is altered by PIP5Pase inactivation or knockdown | Trypanosoma brucei |
3.1.3.86 | physiological function | the enzyme's activity controls 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate binding by RAP1 and, thus, transcriptional silencing of variant surface glycoprotein genes | Trypanosoma brucei |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.36 | 653 | - |
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
3.1.3.36 | 892 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | mutant D360A/N362A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
3.1.3.36 | 1900 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
3.1.3.86 | 653 | - |
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate | wild type enzyme, at pH 7.5 and 25°C | Trypanosoma brucei | |
3.1.3.86 | 892 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | mutant enzyme D360A/N362A, at pH 7.5 and 25°C | Trypanosoma brucei | |
3.1.3.86 | 1900 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | wild type enzyme, at pH 7.5 and 25°C | Trypanosoma brucei |