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Literature summary extracted from

  • Wagschal, K.; Rose Stoller, J.; Chan, V.J.; Lee, C.C.; Grigorescu, A.A.; Jordan, D.B.
    Expression and characterization of hyperthermostable exo-polygalacturonase TtGH28 from Thermotoga thermophilus (2016), Mol. Biotechnol., 58, 509-519 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.67 expressed in Escherichia coli BL21(DE3) CodonPlus cells Thermotoga thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 digalacturonic acid
-
 Thermotoga thermophilus
3.2.1.67 galacturonic acid
-
 Thermotoga thermophilus
3.2.1.67 additional information no substrate inhibition for polygalacturonate Thermotoga thermophilus
3.2.1.67 trigalacturonic acid
-
 Thermotoga thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.67 0.00877
-
 polygalacturonic acid at pH 6.0 and 40°C Thermotoga thermophilus
3.2.1.67 0.0123
-
 trigalacturonic acid at pH 6.0 and 40°C Thermotoga thermophilus
3.2.1.67 0.0193
-
 digalacturonic acid at pH 6.0 and 40°C Thermotoga thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.67 pectin + H2O Thermotoga thermophilus
-
 galacturonic acid + ?
-
 ?
3.2.1.67 pectin + H2O Thermotoga thermophilus DSM 5069
-
 galacturonic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Thermotoga thermophilus
-
-
-
3.2.1.67 Thermotoga thermophilus DSM 5069
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 Ni-NTA resin column chromatography and Superdex 200 gel filtration Thermotoga thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 digalacturonic acid + H2O
-
 Thermotoga thermophilus 2 galacturonic acid
-
 ?
3.2.1.67 digalacturonic acid + H2O
-
 Thermotoga thermophilus DSM 5069 2 galacturonic acid
-
 ?
3.2.1.67 pectin + H2O
-
 Thermotoga thermophilus galacturonic acid + ?
-
 ?
3.2.1.67 pectin + H2O
-
 Thermotoga thermophilus DSM 5069 galacturonic acid + ?
-
 ?
3.2.1.67 polygalacturonic acid + H2O
-
 Thermotoga thermophilus galacturonic acid
-
 ?
3.2.1.67 polygalacturonic acid + H2O
-
 Thermotoga thermophilus DSM 5069 galacturonic acid
-
 ?
3.2.1.67 trigalacturonic acid + H2O
-
 Thermotoga thermophilus digalacturonic acid + galacturonic acid
-
 ?
3.2.1.67 trigalacturonic acid + H2O
-
 Thermotoga thermophilus DSM 5069 digalacturonic acid + galacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 homodimer 2 * 117010, calculated from amino acid sequence Thermotoga thermophilus
3.2.1.67 homodimer 2 * 121510, SDS-PAGE Thermotoga thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 exo-polygalacturonanase
-
 Thermotoga thermophilus
3.2.1.67 GH28
-
 Thermotoga thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 86.3
-
 the enzyme is hyperthermostable with 50% of activity remaining at 86.3°C after 1 h incubation Thermotoga thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.67 9.55
-
 trigalacturonic acid at pH 6.0 and 40°C Thermotoga thermophilus
3.2.1.67 10.6
-
 polygalacturonic acid at pH 6.0 and 40°C Thermotoga thermophilus
3.2.1.67 14.1
-
 digalacturonic acid at pH 6.0 and 40°C Thermotoga thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.67 0.0079
-
 galacturonic acid at pH 6.0 and 40°C Thermotoga thermophilus
3.2.1.67 3.63
-
 digalacturonic acid at pH 6.0 and 40°C Thermotoga thermophilus
3.2.1.67 17.2
-
 trigalacturonic acid at pH 6.0 and 40°C Thermotoga thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.67 731
-
 digalacturonic acid at pH 6.0 and 40°C Thermotoga thermophilus
3.2.1.67 776
-
 trigalacturonic acid at pH 6.0 and 40°C Thermotoga thermophilus
3.2.1.67 1209
-
 polygalacturonic acid at pH 6.0 and 40°C Thermotoga thermophilus