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Literature summary extracted from

  • Espinosa-Luna, G.; Sanchez-Otero, M.G.; Quintana-Castro, R.; Matus-Toledo, R.E.; Oliart-Ros, R.M.
    Gene cloning and characterization of the Geobacillus thermoleovorans CCR11 carboxylesterase CaesCCR11, a new member of family XV (2016), Mol. Biotechnol., 58, 37-46 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.1 2-mercaptoethanol 1% v/v, 141.5% activity Geobacillus thermoleovorans
3.1.1.1 EDTA 1 mM, 143.2% activity Geobacillus thermoleovorans
3.1.1.1 Triton X-100 1% v/v, 136.9% activity Geobacillus thermoleovorans
3.1.1.1 Tween 20 1% v/v, 121.9% activity Geobacillus thermoleovorans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 gene caesCCR11, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli strain BL21(DE3) Geobacillus thermoleovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 acetone
-
 Geobacillus thermoleovorans
3.1.1.1 butanol
-
 Geobacillus thermoleovorans
3.1.1.1 dioxane
-
 Geobacillus thermoleovorans
3.1.1.1 ethanol
-
 Geobacillus thermoleovorans
3.1.1.1 ethyl acetate
-
 Geobacillus thermoleovorans
3.1.1.1 heptane
-
 Geobacillus thermoleovorans
3.1.1.1 hexane
-
 Geobacillus thermoleovorans
3.1.1.1 Hg2+ 90% inhibition Geobacillus thermoleovorans
3.1.1.1 K+ 10% inhibition Geobacillus thermoleovorans
3.1.1.1 methanol
-
 Geobacillus thermoleovorans
3.1.1.1 PMSF 1 mM, 85% inhibition Geobacillus thermoleovorans
3.1.1.1 Propanol
-
 Geobacillus thermoleovorans
3.1.1.1 SDS 0.1% w/v, 24.2% inhibition Geobacillus thermoleovorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.1 extracellular
-
 Geobacillus thermoleovorans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 Ba2+ activates slightly Geobacillus thermoleovorans
3.1.1.1 Li+ activates slightly Geobacillus thermoleovorans
3.1.1.1 Mg2+ activates slightly Geobacillus thermoleovorans
3.1.1.1 Na+ activates slightly Geobacillus thermoleovorans

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.1 Acetone 21.8% residual activity after 1 h Geobacillus thermoleovorans
3.1.1.1 Butanol 22.9% residual activity after 1 h Geobacillus thermoleovorans
3.1.1.1 dioxane 13.2% residual activity after 1 h Geobacillus thermoleovorans
3.1.1.1 Ethanol 27.5% residual activity after 1 h Geobacillus thermoleovorans
3.1.1.1 Ethyl acetate 24.8% residual activity after 1 h Geobacillus thermoleovorans
3.1.1.1 heptane 74.6% residual activity after 1 h Geobacillus thermoleovorans
3.1.1.1 hexane 59.2% residual activity after 1 h Geobacillus thermoleovorans
3.1.1.1 Methanol 12.7% residual activity after 1 h Geobacillus thermoleovorans
3.1.1.1 propanol 13.1% residual activity after 1 h Geobacillus thermoleovorans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Geobacillus thermoleovorans W8QMJ2 isolated from El Carrizal hot springs in Veracruz, Mexico
-
3.1.1.1 Geobacillus thermoleovorans CCR11 W8QMJ2 isolated from El Carrizal hot springs in Veracruz, Mexico
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 native extracellular enzyme and recombinant enzyme by ultrafiltration Geobacillus thermoleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 1375.7
-
 purified recombinant enzyme, pH 6.5, 30°C, substrate 4-nitrophenyl laurate Geobacillus thermoleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl butyrate + H2O best substrate Geobacillus thermoleovorans 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O best substrate Geobacillus thermoleovorans CCR11 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl caprate + H2O
-
 Geobacillus thermoleovorans 4-nitrophenol + caprate
-
 ?
3.1.1.1 4-nitrophenyl caprate + H2O
-
 Geobacillus thermoleovorans CCR11 4-nitrophenol + caprate
-
 ?
3.1.1.1 4-nitrophenyl caproate + H2O
-
 Geobacillus thermoleovorans 4-nitrophenol + caproate
-
 ?
3.1.1.1 4-nitrophenyl caproate + H2O
-
 Geobacillus thermoleovorans CCR11 4-nitrophenol + caproate
-
 ?
3.1.1.1 additional information enzyme CaesCCR11 has a preference for C4 substrates. Activity toward 4-nitrophenyl esters with longer chain lengths is only slightly detected, suggesting that CaesCCR11 is an esterase Geobacillus thermoleovorans ?
-
 ?
3.1.1.1 additional information enzyme CaesCCR11 has a preference for C4 substrates. Activity toward 4-nitrophenyl esters with longer chain lengths is only slightly detected, suggesting that CaesCCR11 is an esterase Geobacillus thermoleovorans CCR11 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 27300, about, sequence calculation Geobacillus thermoleovorans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 CaesCCR11
-
 Geobacillus thermoleovorans
3.1.1.1 More cf. EC 3.1.1.3 Geobacillus thermoleovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 50
-
 recombinant enzyme Geobacillus thermoleovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 30 80 purified recombinant enzyme, at 30°C and 40°C, lipolytic activity is conserved after 30 and 20 min, respectively. When the enzyme is preincubated around the physiological temperature at 50-60°C for 30 min, lipolytic activity is increased by 80%, it is diminished by 50% at 70°C and completely lost at 80°C after 10 min Geobacillus thermoleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 5 8 broad optimum, recombinant enzyme Geobacillus thermoleovorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.1 6 8 purified recombinant enzyme, the activity is retained. Lipolytic activity is increased to 150% after a 28 h incubation at pH 9.0 and pH 10.0, while activity is lost by 50% at pH 5.0 and pH 11.0 Geobacillus thermoleovorans

General Information

EC Number General Information Comment Organism
3.1.1.1 evolution CaesCCR11 as a member of family XV of lipolytic enzymes, it has a canonical alpha/beta hydrolase fold composed of 7 beta-strands and 6 alpha-helices, the alpha/beta architecture of the cap domain, the GLSTG pentapeptide, and the formation of distinctive salt bridges Geobacillus thermoleovorans
3.1.1.1 additional information three-dimensional structure modeling of enzyme CaesCCR11 using the Bacillus sp. monoacyl glycerol lipase bMGL H-257 structure as template (PBD ID 3RM3) Geobacillus thermoleovorans