Any feedback?
Literature summary extracted from
- Expression and characterisation of a thermophilic endo-1,4-beta-glucanase from Sulfolobus shibatae of potential industrial application (2018), Mol. Biol. Rep., 45, 2201-2211 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | industry | the thermophilic nature and biochemical properties of the enzyme indicate its potential suitability in industrial applications undertaken at high temperature, such as the production of second-generation bioethanol from lignocellulosic feedstocks and in the brewing industry | Saccharolobus shibatae |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | gene ssgluc, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain Rosetta-gami B (DE3), subcloning in Escherichia coli strain DH5alpha | Saccharolobus shibatae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | Saccharolobus shibatae | A0A1B5G0A1 | - |
- |
| 3.2.1.4 | Saccharolobus shibatae B12 | A0A1B5G0A1 | - |
- |
| 3.2.1.4 | Saccharolobus shibatae DSM 5389 | A0A1B5G0A1 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | recombinant N-terminally His-tagged enzyme 2535fold from Escherichia coli by nickel affinity chromatography and ultrafiltration | Saccharolobus shibatae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 428 | - |
purified recombinant His-tagged enzyme, pH 4.0, 95°C, substrate carboxymethyl cellulose | Saccharolobus shibatae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.4 | barley beta-glucan + H2O | best substrate | Saccharolobus shibatae | ? | - |
? |  |
| 3.2.1.4 | barley beta-glucan + H2O | best substrate | Saccharolobus shibatae B12 | ? | - |
? | |
| 3.2.1.4 | barley beta-glucan + H2O | best substrate | Saccharolobus shibatae DSM 5389 | ? | - |
? | |
| 3.2.1.4 | carboxymethyl cellulose + H2O | - |
Saccharolobus shibatae | ? | - |
? | |
| 3.2.1.4 | carboxymethyl cellulose + H2O | - |
Saccharolobus shibatae B12 | ? | - |
? | |
| 3.2.1.4 | carboxymethyl cellulose + H2O | - |
Saccharolobus shibatae DSM 5389 | ? | - |
? | |
| 3.2.1.4 | lichenan + H2O | best substrate | Saccharolobus shibatae | ? | - |
? | |
| 3.2.1.4 | lichenan + H2O | best substrate | Saccharolobus shibatae B12 | ? | - |
? | |
| 3.2.1.4 | lichenan + H2O | best substrate | Saccharolobus shibatae DSM 5389 | ? | - |
? | |
| 3.2.1.4 | additional information | the crude enzyme releases reducing sugars from acid-pretreated straw at 75-85°C | Saccharolobus shibatae | ? | - |
? | |
| 3.2.1.4 | additional information | the crude enzyme releases reducing sugars from acid-pretreated straw at 75-85°C | Saccharolobus shibatae B12 | ? | - |
? | |
| 3.2.1.4 | additional information | the crude enzyme releases reducing sugars from acid-pretreated straw at 75-85°C | Saccharolobus shibatae DSM 5389 | ? | - |
? | |
| 3.2.1.4 | xylan + H2O | - |
Saccharolobus shibatae | ? | - |
? | |
| 3.2.1.4 | xylan + H2O | - |
Saccharolobus shibatae B12 | ? | - |
? | |
| 3.2.1.4 | xylan + H2O | - |
Saccharolobus shibatae DSM 5389 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | ? | x * 35128, sequence calculation | Saccharolobus shibatae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | endo-1,4-B-glucanase | UniProt | Saccharolobus shibatae |
| 3.2.1.4 | endo-1,4-beta-glucanase | - |
Saccharolobus shibatae |
| 3.2.1.4 | endo-beta-glucanase | - |
Saccharolobus shibatae |
| 3.2.1.4 | endoglucanase | - |
Saccharolobus shibatae |
| 3.2.1.4 | ssgluc | - |
Saccharolobus shibatae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 95 | 100 | recombinant enzyme | Saccharolobus shibatae |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 75 | 110 | recombinant enzyme, over 20% of maximal activity within this range, 50% at 80°C and 105°C, profile overview | Saccharolobus shibatae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 75 | - |
purified recombinant enzyme, completely stable for 1 h, and 98% activity remaining after 2 h | Saccharolobus shibatae |
| 3.2.1.4 | 80 | - |
purified recombinant enzyme, completely stable for 1 h, and 90% activity remaining after 2 h | Saccharolobus shibatae |
| 3.2.1.4 | 85 | - |
purified recombinant enzyme, completely stable for 1 h, and 84% activity remaining after 2 h | Saccharolobus shibatae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 4 | - |
recombinant enzyme | Saccharolobus shibatae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 3 | 5 | recombinant enzyme, over 91% of maximal activity within this range, pH profile, overview | Saccharolobus shibatae |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.4 | Saccharolobus shibatae | sequence calculation | - |
4.98 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
