Literature summary extracted from

Zhang, X.; Liu, Q.; Jin, Y.; Li, B.
Determination of the activity of T4 polynucleotide kinase phosphatase by exploiting the sequence-dependent fluorescence of DNA-templated copper nanoclusters (2019), Microchim. Acta, 186, 003 .

Application

EC Number	Application	Comment	Organism
2.7.1.78	analysis	fluorometric method for the determination of the activity of T4 polynucleotide kinase phosphatase PNKP. A partially doublestranded DNA substrate is dephosphorylated on addition of T4 PNKP to generate the long dsDNA, which acts as a template for synthesizing copper nanoclusters. The assay has an analytical range from 0.07 U/ml to 15 U/ml and a detection limit of 0.06 U/ml	Tequatrovirus T4
3.1.3.32	analysis	fluorometric method for the determination of the activity of T4 polynucleotide kinase phosphatase PNKP. A partially doublestranded DNA substrate is dephosphorylated on addition of T4 PNKP to generate the long dsDNA, which acts as a template for synthesizing copper nanoclusters. The assay has an analytical range from 0.07 U/ml to 15 U/ml and a detection limit of 0.06 U/ml	Tequatrovirus T4

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.1.78	Tequatrovirus T4	P06855	-	-
3.1.3.32	Tequatrovirus T4	P06855	cf. 2.7.1.78	-

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)