EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.48 | gene SPD_1837, recombinant expression in Escherichia coli strain BL21(DE3) | Streptococcus pneumoniae D39 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.48 | C8S | site-directed mutagenesis, mutation in the putative active site cysteine, almost inactive enzyme mutant | Streptococcus pneumoniae D39 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.48 | iodoacetic acid | - |
Streptococcus pneumoniae D39 | |
3.1.3.48 | sodium orthovanadate | - |
Streptococcus pneumoniae D39 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.48 | additional information | - |
additional information | Michaelis-Menten kinetics | Streptococcus pneumoniae D39 | |
3.1.3.48 | 8 | - |
4-nitrophenyl phosphate | recombinant enzyme, pH 7.0, 37°C | Streptococcus pneumoniae D39 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.48 | 15800 | - |
recombinant enzyme, gel filtration | Streptococcus pneumoniae D39 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.48 | [a protein]-tyrosine phosphate + H2O | Streptococcus pneumoniae D39 | - |
[a protein]-tyrosine + phosphate | - |
? | |
3.1.3.48 | [a protein]-tyrosine phosphate + H2O | Streptococcus pneumoniae D39 NCTC 7466 | - |
[a protein]-tyrosine + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.48 | Streptococcus pneumoniae D39 | A0A0H2ZQ64 | - |
- |
3.1.3.48 | Streptococcus pneumoniae D39 NCTC 7466 | A0A0H2ZQ64 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.48 | 4-nitrophenyl phosphate + H2O | - |
Streptococcus pneumoniae D39 | 4-nitrophenol + phosphate | - |
? | |
3.1.3.48 | 4-nitrophenyl phosphate + H2O | - |
Streptococcus pneumoniae D39 NCTC 7466 | 4-nitrophenol + phosphate | - |
? | |
3.1.3.48 | DADE(pY)LIPQQG + H2O | phosphopeptide-2 | Streptococcus pneumoniae D39 | DADEYLIPQQG + phosphate | - |
? | |
3.1.3.48 | DADE(pY)LIPQQG + H2O | phosphopeptide-2 | Streptococcus pneumoniae D39 NCTC 7466 | DADEYLIPQQG + phosphate | - |
? | |
3.1.3.48 | END(pY)INASL + H2O | phosphopeptide-1 | Streptococcus pneumoniae D39 | ENDYINASL + phosphate | - |
? | |
3.1.3.48 | END(pY)INASL + H2O | phosphopeptide-1 | Streptococcus pneumoniae D39 NCTC 7466 | ENDYINASL + phosphate | - |
? | |
3.1.3.48 | additional information | Spd1837 has activity against two phosphotyrosine-containing peptides, phosphopeptide-1 (END(pY)INASL) and phosphopeptide-2 (DADE(pY)LIPQQG), as the mutant protein Spd1837C8S lacks activity against these two phosphotyrosine-containing phosphopeptides | Streptococcus pneumoniae D39 | ? | - |
? | |
3.1.3.48 | additional information | Spd1837 has activity against two phosphotyrosine-containing peptides, phosphopeptide-1 (END(pY)INASL) and phosphopeptide-2 (DADE(pY)LIPQQG), as the mutant protein Spd1837C8S lacks activity against these two phosphotyrosine-containing phosphopeptides | Streptococcus pneumoniae D39 NCTC 7466 | ? | - |
? | |
3.1.3.48 | [a protein]-tyrosine phosphate + H2O | - |
Streptococcus pneumoniae D39 | [a protein]-tyrosine + phosphate | - |
? | |
3.1.3.48 | [a protein]-tyrosine phosphate + H2O | - |
Streptococcus pneumoniae D39 NCTC 7466 | [a protein]-tyrosine + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.48 | monomer | 1 * 15800, recombinant enzyme, SDS-PAGE | Streptococcus pneumoniae D39 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.48 | LMWPTP | - |
Streptococcus pneumoniae D39 |
3.1.3.48 | low molecular weight protein tyrosine phosphatase | - |
Streptococcus pneumoniae D39 |
3.1.3.48 | protein tyrosine phosphatase | - |
Streptococcus pneumoniae D39 |
3.1.3.48 | PTP | - |
Streptococcus pneumoniae D39 |
3.1.3.48 | Spd1837 | - |
Streptococcus pneumoniae D39 |
3.1.3.48 | SPD_1837 | - |
Streptococcus pneumoniae D39 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.48 | 37 | - |
assay at | Streptococcus pneumoniae D39 |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.48 | 30 | 50 | activity range, profile overview | Streptococcus pneumoniae D39 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.48 | 7 | - |
assay at | Streptococcus pneumoniae D39 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.48 | 5 | 9.5 | activity range, profile overview | Streptococcus pneumoniae D39 |