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Literature summary extracted from

  • Ahmad, Z.; Morona, R.; Standish, A.J.
    In vitro characterization and identification of potential substrates of a low molecular weight protein tyrosine phosphatase in Streptococcus pneumoniae (2018), Microbiology, 164, 697-703 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.48 gene SPD_1837, recombinant expression in Escherichia coli strain BL21(DE3) Streptococcus pneumoniae D39

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.48 C8S site-directed mutagenesis, mutation in the putative active site cysteine, almost inactive enzyme mutant Streptococcus pneumoniae D39

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.48 iodoacetic acid
-
Streptococcus pneumoniae D39
3.1.3.48 sodium orthovanadate
-
Streptococcus pneumoniae D39

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.48 additional information
-
additional information Michaelis-Menten kinetics Streptococcus pneumoniae D39
3.1.3.48 8
-
4-nitrophenyl phosphate recombinant enzyme, pH 7.0, 37°C Streptococcus pneumoniae D39

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.48 15800
-
recombinant enzyme, gel filtration Streptococcus pneumoniae D39

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.48 [a protein]-tyrosine phosphate + H2O Streptococcus pneumoniae D39
-
[a protein]-tyrosine + phosphate
-
?
3.1.3.48 [a protein]-tyrosine phosphate + H2O Streptococcus pneumoniae D39 NCTC 7466
-
[a protein]-tyrosine + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.48 Streptococcus pneumoniae D39 A0A0H2ZQ64
-
-
3.1.3.48 Streptococcus pneumoniae D39 NCTC 7466 A0A0H2ZQ64
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.48 4-nitrophenyl phosphate + H2O
-
Streptococcus pneumoniae D39 4-nitrophenol + phosphate
-
?
3.1.3.48 4-nitrophenyl phosphate + H2O
-
Streptococcus pneumoniae D39 NCTC 7466 4-nitrophenol + phosphate
-
?
3.1.3.48 DADE(pY)LIPQQG + H2O phosphopeptide-2 Streptococcus pneumoniae D39 DADEYLIPQQG + phosphate
-
?
3.1.3.48 DADE(pY)LIPQQG + H2O phosphopeptide-2 Streptococcus pneumoniae D39 NCTC 7466 DADEYLIPQQG + phosphate
-
?
3.1.3.48 END(pY)INASL + H2O phosphopeptide-1 Streptococcus pneumoniae D39 ENDYINASL + phosphate
-
?
3.1.3.48 END(pY)INASL + H2O phosphopeptide-1 Streptococcus pneumoniae D39 NCTC 7466 ENDYINASL + phosphate
-
?
3.1.3.48 additional information Spd1837 has activity against two phosphotyrosine-containing peptides, phosphopeptide-1 (END(pY)INASL) and phosphopeptide-2 (DADE(pY)LIPQQG), as the mutant protein Spd1837C8S lacks activity against these two phosphotyrosine-containing phosphopeptides Streptococcus pneumoniae D39 ?
-
?
3.1.3.48 additional information Spd1837 has activity against two phosphotyrosine-containing peptides, phosphopeptide-1 (END(pY)INASL) and phosphopeptide-2 (DADE(pY)LIPQQG), as the mutant protein Spd1837C8S lacks activity against these two phosphotyrosine-containing phosphopeptides Streptococcus pneumoniae D39 NCTC 7466 ?
-
?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
Streptococcus pneumoniae D39 [a protein]-tyrosine + phosphate
-
?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
Streptococcus pneumoniae D39 NCTC 7466 [a protein]-tyrosine + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.48 monomer 1 * 15800, recombinant enzyme, SDS-PAGE Streptococcus pneumoniae D39

Synonyms

EC Number Synonyms Comment Organism
3.1.3.48 LMWPTP
-
Streptococcus pneumoniae D39
3.1.3.48 low molecular weight protein tyrosine phosphatase
-
Streptococcus pneumoniae D39
3.1.3.48 protein tyrosine phosphatase
-
Streptococcus pneumoniae D39
3.1.3.48 PTP
-
Streptococcus pneumoniae D39
3.1.3.48 Spd1837
-
Streptococcus pneumoniae D39
3.1.3.48 SPD_1837
-
Streptococcus pneumoniae D39

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.48 37
-
assay at Streptococcus pneumoniae D39

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.48 30 50 activity range, profile overview Streptococcus pneumoniae D39

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.48 7
-
assay at Streptococcus pneumoniae D39

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.48 5 9.5 activity range, profile overview Streptococcus pneumoniae D39