Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Roenneke, B.; Rosenfeldt, N.; Derya, S.M.; Novak, J.F.; Marin, K.; Kraemer, R.; Seibold, G.M.
    Production of the compatible solute alpha-D-glucosylglycerol by metabolically engineered Corynebacterium glutamicum (2018), Microb. Cell Fact., 17, 94 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.3.69 synthesis plasmid-bound expression of glucosylglycerol synthase and glucosylglycerol phosphatase from Synechocystis sp. PCC 6803 enables alpha-D-glucosylglycerol synthesis exclusively in osmotically stressed cells of Corynebacterium glutamicum. Upon deletion of both the glgA encoded glycogen synthase and the otsA encoded trehalose-6-phosphate synthase, the alpha-D-glucosylglycerol concentration in culture supernatants is increased from 0.5 mM in to 2.9 mM. Upon nitrogen limitation, transgenic Corynebacterium produces more than 10 mM alpha-D-glucosylglycerol (about 2 g/l) Synechocystis sp. PCC 6803

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.213 plasmid-bound expression of ggpS and ggpP from Synechocystis sp. PCC 6803 enables synthesis of alpha-D-glucosylglycerol exclusively in osmotically stressed cells of Corynebacterium glutamicum (pEKEx2-ggpSP), which is probably due to the unique intrinsic control mechanism of GG-phosphate synthase activity in response to intracellular ion concentrations Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.213 ADP-alpha-D-glucose + sn-glycerol 3-phosphate Synechocystis sp. PCC 6803
-
 2-(alpha-D-glucopyranosyl)-sn-glycerol 3-phosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.213 Synechocystis sp. PCC 6803 P74258
-
-
3.1.3.69 Synechocystis sp. PCC 6803 Q55034
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.213 ADP-alpha-D-glucose + sn-glycerol 3-phosphate
-
 Synechocystis sp. PCC 6803 2-(alpha-D-glucopyranosyl)-sn-glycerol 3-phosphate + ADP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.213 GG-phosphate synthase
-
 Synechocystis sp. PCC 6803
2.4.1.213 GGPS
-
 Synechocystis sp. PCC 6803