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Literature summary extracted from

  • See-Too, W.S.; Convey, P.; Pearce, D.A.; Chan, K.G.
    Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp (2018), Microb. Cell Fact., 17, 179 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 expression in Escherichia coli BL21 StarTM cells Planococcus versutus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.81 Ca2+ 1 mM, about 30% inhibition, in vivo and in vitro assay Planococcus versutus
3.1.1.81 Cr3+ 1 mM, 30% inhibition in the in vitro assay, about 20% inhibition in the in vivo assay Planococcus versutus
3.1.1.81 Cu2+ 1 mM, 45% inhibition in the in vitro assay, about 50% inhibition in the in vivo assay Planococcus versutus
3.1.1.81 EDTA
-
 Planococcus versutus
3.1.1.81 Fe2+ 1 mM, about 30% inhibition, in vivo and in vitro assay Planococcus versutus
3.1.1.81 Mg2+ 1 mM, 90% inhibition in the in vitro assay, about 30% inhibition in the in vivo assay Planococcus versutus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.81 Zn2+ 1 mM, 1.45fold activation in the in vivo assay Planococcus versutus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Planococcus versutus
-
-
-
3.1.1.81 Planococcus versutus L10.15T
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.81
-
 Planococcus versutus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O about 60% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-3-oxododecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O about 60% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus L10.15T N-3-oxododecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O best substrate Planococcus versutus N-3-oxohexanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O best substrate Planococcus versutus L10.15T N-3-oxohexanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxooctanoyl-L-homoserine lactone + H2O about 80% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-3-oxooctanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxooctanoyl-L-homoserine lactone + H2O about 80% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus L10.15T N-3-oxooctanoyl-L-homoserine
-
 ?
3.1.1.81 N-butyryl-L-homoserine lactone + H2O about 10% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-butyryl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O about 40% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O about 40% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus L10.15T N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-heptanoyl-L-homoserine lactone + H2O about 10% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-heptanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O about 90% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O about 90% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus L10.15T N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-octanoyl-L-homoserine lactone + H2O about 55% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-octanoyl-L-homoserine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 AHL lactonase
-
 Planococcus versutus
3.1.1.81 AidP
-
 Planococcus versutus
3.1.1.81 N-acylhomoserine lactonase
-
 Planococcus versutus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.81 28
-
-
 Planococcus versutus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.81 17 43 17°C: about 60% of maximal activity, 43°C: about 40% of maximal activity Planococcus versutus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.81 32
-
 3 h, about 35% loss of activity Planococcus versutus
3.1.1.81 37
-
 3 h, about 75% loss of activity Planococcus versutus
3.1.1.81 40
-
 3 h, about 80% loss of activity Planococcus versutus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.81 8 9
-
 Planococcus versutus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.81 5 9 pH 5.0: about 50% of maximal activity, pH 8.0-9.0: maximal activity Planococcus versutus