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Literature summary extracted from

  • Liu, X.; Liang, M.; Liu, Y.; Fan, X.
    Directed evolution and secretory expression of a pyrethroid-hydrolyzing esterase with enhanced catalytic activity and thermostability (2017), Microb. Cell Fact., 16, 81 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.1.88 environmental protection the mutant enzyme A171V/D256N is an ideal candidate for the biodegradation of pyrethroids (widely used as insecticides) uncultured bacterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.88 extracellularly expressed in a Pichia pastoris host system uncultured bacterium

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.88 A171V the mutant displays 2.46fold higher catalytic activity with the substrate 4-nitrophenyl acetate uncultured bacterium
3.1.1.88 A171V/D256N the mutant displays 2.46fold higher catalytic activity with the substrate 4-nitrophenyl acetate. The mutant is able to efficiently degrade all the pyrethroids (cyhalothrin, cypermethrin, sumicidin, deltamethrin) tested within a short time, indicating that the mutant possesses broad substrate specificity. It shows a broad substrate specificity. The optimal temperature is 10°C higher than that of the wild-type enzyme (55°C). The half-life at 40-65°C is 3.3-310 times longer. The mutant enzyme has a half-life of 12 h at 70°C while the wild-type Sys410 is completely inactivated at 70°C uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.88 0.01161
-
 4-nitrophenyl acetate mutant enzyme A171V/D256N, pH 6.5, 55°C uncultured bacterium
3.1.1.88 0.0137
-
 4-nitrophenyl acetate mutant enzyme A171V, pH 6.5, 55°C uncultured bacterium
3.1.1.88 0.1411
-
 4-nitrophenyl acetate wild-type enzyme, pH 6.5, 55°C uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.88 uncultured bacterium H9CDG2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.88
-
 uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.88 4-nitrophenyl acetate + H2O best substrate for wild-type end mutant enzymes A171V and A171V/D256N uncultured bacterium 4-nitrophenol + acetate
-
 ?
3.1.1.88 4-nitrophenyl butyrate + H2O
-
 uncultured bacterium 4-nitrophenol + butyrate
-
 ?
3.1.1.88 4-nitrophenyl decanoate + H2O low activity, wild-type and mutant enzymes A171V and A171V/D256N uncultured bacterium 4-nitrophenol + decanoate
-
 ?
3.1.1.88 4-nitrophenyl dodecanoate + H2O low activity, wild-type and mutant enzymes A171V and A171V/D256N uncultured bacterium 4-nitrophenol + dodecanoate
-
 ?
3.1.1.88 4-nitrophenyl hexanoate + H2O
-
 uncultured bacterium 4-nitrophenol + hexanoate
-
 ?
3.1.1.88 4-nitrophenyl octanoate + H2O low activity, wild-type and mutant enzymes A171V and A171V/D256N uncultured bacterium 4-nitrophenol + octanoate
-
 ?
3.1.1.88 cyhalothrin + H2O
-
 uncultured bacterium 3-phenoxybenzaldehyde + 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
3.1.1.88 cypermethrin + H2O
-
 uncultured bacterium 3-phenoxybenzaldehyde + 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
3.1.1.88 deltamethrin + H2O
-
 uncultured bacterium 3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
3.1.1.88 sumicidin + H2O
-
 uncultured bacterium 3-phenoxybenzaldehyde + 2-(4-chlorophenyl)-3-methylbutanoic acid + cyanide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.88 esterase sys410
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.88 50
-
 wild-type enzyme uncultured bacterium
3.1.1.88 65
-
 mutant enzymes A171V and A171V/D256N uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.88 35 65 35°C: about 75% of maximal activity, 65°C: about 65% of maximal activity, wild-type enzyme uncultured bacterium
3.1.1.88 40 80 40°C: about 55% of maximal activity, 80°C: about 60% of maximal activity, mutant enzyme A171V/D256N uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.88 40
-
 T1/2: 72 h (wild-type enzyme Sys410), 236 h (mutant enzyme A171V/D256N) uncultured bacterium
3.1.1.88 45
-
 T1/2: 38 h (wild-type enzyme Sys410), 154 h (mutant enzyme A171V/D256N) uncultured bacterium
3.1.1.88 50
-
 T1/2: 10 h (wild-type enzyme Sys410), 133 h (mutant enzyme A171V/D256N) uncultured bacterium
3.1.1.88 55
-
 T1/2: 6 h (wild-type enzyme Sys410), 77 h (mutant enzyme A171V/D256N) uncultured bacterium
3.1.1.88 60
-
 T1/2: 84 min (wild-type enzyme Sys410), 53 h (mutant enzyme A171V/D256N) uncultured bacterium
3.1.1.88 65
-
 T1/2: 6 min (wild-type enzyme Sys410), 31 h (mutant enzyme A171V/D256N) uncultured bacterium
3.1.1.88 70
-
 T1/2: 12 h (mutant enzyme A171V/D256N) uncultured bacterium
3.1.1.88 80
-
 T1/2: 4 h (mutant enzyme A171V/D256N) uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.88 289.12
-
 4-nitrophenyl acetate wild-type enzyme, pH 6.5, 55°C uncultured bacterium
3.1.1.88 293.31
-
 4-nitrophenyl acetate mutant enzyme A171V, pH 6.5, 55°C uncultured bacterium
3.1.1.88 350.15
-
 4-nitrophenyl acetate mutant enzyme A171V/D256N, pH 6.5, 55°C uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.88 5.5 6.5 wild-type enzyme uncultured bacterium
3.1.1.88 7
-
 mutant enzymes A171V and A171V/D256N uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.88 4.5 8.5 pH 4.5: about 50% of maximal activity, pH 8.5: about 50% of maximal activity, mutant enzyme A171V/D256N uncultured bacterium
3.1.1.88 4.5 8.5 pH 4.5: about 65% of maximal activity, pH 8.5: about 60% of maximal activity, wild-type enzyme uncultured bacterium