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Literature summary extracted from

  • Carrasco, M.; Alcaino, J.; Cifuentes, V.; Baeza, M.
    Purification and characterization of a novel cold adapted fungal glucoamylase (2017), Microb. Cell Fact., 16, 75 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 DNA and amino acid sequence determination, analysis and comparisons Tetracladium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information 4.5 g/l with soluble starch Tetracladium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.3 extracellular the enzyme is secreted Tetracladium sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.3 additional information the enzyme does not depend on Ca2+ for its hydrolytic activity Tetracladium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Tetracladium sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 starch + H2O soluble starch Tetracladium sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 More enzyme structure modelling and comparison of the structural model of Tetracladium sp. glucoamylase with the solved structure of the Hypocrea jecorina glucoamylase Tetracladium sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Tetracladium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 30
-
-
 Tetracladium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.3 additional information
-
 glucoamylase secreted by Tetracladium sp. is a novel cold-adapted enzyme Tetracladium sp.
3.2.1.3 4 60 at 22°C and 4°C, the enzyme shows 55% and 25% of its maximal activity, lower activity above 45°C, highest activity between 30°C and 45°C Tetracladium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 22 30 purified enzyme, pH 6.0, 6-8 h, stable Tetracladium sp.
3.2.1.3 40
-
 purified enzyme, pH 6.0, loss of 30% activity after 2 h, loss of 60% activity after 8 h Tetracladium sp.
3.2.1.3 50
-
 purified enzyme, pH 6.0, 1 h, loss of 90% activity Tetracladium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 0.75
-
 starch soluble starch, pH 6.0, 30°C Tetracladium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 6
-
-
 Tetracladium sp.

General Information

EC Number General Information Comment Organism
3.2.1.3 additional information enzyme structure modelling and comparison of the structural model of Tetracladium sp. glucoamylase with the solved structure of the Hypocrea jecorina glucoamylase Tetracladium sp.