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Literature summary extracted from
- Identification and immobilization of a novel cold-adapted esterase, and its potential for bioremediation of pyrethroid-contaminated vegetables (2017), Microb. Cell Fact., 16, 149 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.1.88 | food industry | high potential for bioremediation of pyrethroid-contaminated vegetables | uncultured microorganism |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.88 | expressed in Escherichia coli BL21 (DE3) in soluble form | uncultured microorganism |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.1.88 | high reusability, the immobilized Est684 (immobilized on on mesoporous silica) retains nearly 54% of its activity after 28 cycles, indicating excellent operational stability | uncultured microorganism |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.88 | 0.00601 | - |
4-nitrophenyl butyrate | pH 6.5, 18°C, soluble enzyme | uncultured microorganism |  |
| 3.1.1.88 | 0.00792 | - |
4-nitrophenyl butyrate | pH 6.5, 18°C, immobilized enzyme | uncultured microorganism | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.88 | 35000 | - |
below, SDS-PAGE | uncultured microorganism |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.88 | uncultured microorganism | A0A290U7B6 | gene (est684) isolated from the Mao-tofu metagenome | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.88 | - |
uncultured microorganism |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.88 | 4-nitrophenyl acetate + H2O | about 70% of the activity with 4-nitrophenyl butyrate | uncultured microorganism | 4-nitrophenol + acetate | - |
? | |
| 3.1.1.88 | 4-nitrophenyl butyrate + H2O | highest activity towards 4-nitrophenyl butyrate among all tested 4-nitrophenyl esters | uncultured microorganism | 4-nitrophenol + butyrate | - |
? | |
| 3.1.1.88 | 4-nitrophenyl decanoate + H2O | about 10% of the activity with 4-nitrophenyl butyrate | uncultured microorganism | 4-nitrophenol + decanoate | - |
? | |
| 3.1.1.88 | 4-nitrophenyl dodecanoate + H2O | about 5% of the activity with 4-nitrophenyl butyrate | uncultured microorganism | 4-nitrophenol + dodecanoate | - |
? | |
| 3.1.1.88 | 4-nitrophenyl hexanoate + H2O | about 85% of the activity with 4-nitrophenyl butyrate | uncultured microorganism | 4-nitrophenol + hexanoate | - |
? | |
| 3.1.1.88 | 4-nitrophenyl octanoate + H2O | about 35% of the activity with 4-nitrophenyl butyrate | uncultured microorganism | 4-nitrophenol + octanoate | - |
? | |
| 3.1.1.88 | cyhalothrin + H2O | - |
uncultured microorganism | 3-phenoxybenzaldehyde + 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylate + cyanide | - |
? | |
| 3.1.1.88 | cypermethrin + H2O | - |
uncultured microorganism | 3-phenoxybenzaldehyde + 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide | - |
? | |
| 3.1.1.88 | fenvalerate + H2O | - |
uncultured microorganism | 3-phenoxybenzaldehyde + 2-(4-chlorophenyl)-3-methylbutanoic acid + cyanide | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.88 | ? | x * 35000, SDS-PAGE | uncultured microorganism |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.88 | est684 | - |
uncultured microorganism |
| 3.1.1.88 | pyrethroid-hydrolyzing esterase | - |
uncultured microorganism |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.88 | 18 | - |
soluble and immobilized enzyme | uncultured microorganism |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.88 | 5 | 45 | 5°C: about 60% of maximal activity, 45°C: about 50% of maximal activity, immobilized enzyme | uncultured microorganism |
| 3.1.1.88 | 8 | 40 | 8°C: about 55% of maximal activity, 40°C: about 40% of maximal activity, soluble enzyme | uncultured microorganism |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.88 | 18 | - |
52% residual activity after incubation for 18 h | uncultured microorganism |
| 3.1.1.88 | 35 | - |
t1/2: 6 h ( immobilized enzyme), 1.5 h (soluble enzyme) | uncultured microorganism |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.88 | 403.47 | - |
4-nitrophenyl butyrate | pH 6.5, 18°C, immobilized enzyme | uncultured microorganism | |
| 3.1.1.88 | 417.15 | - |
4-nitrophenyl butyrate | pH 6.5, 18°C, soluble enzyme | uncultured microorganism | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.88 | 6.5 | 7 | immobilized enzyme | uncultured microorganism |
| 3.1.1.88 | 7 | - |
soluble enzyme | uncultured microorganism |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.88 | 5.5 | 8 | the relative activity of the immobilized enzyme is more than 75% at the pH range between 5.5 and 8.0, while the soluble enzyme attains activity over 50% at the same pH range | uncultured microorganism |
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