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Literature summary extracted from

  • Doan, C.T.; Tran, T.N.; Nguyen, V.B.; Nguyen, A.D.; Wang, S.L.
    Production of a thermostable chitosanase from shrimp heads via Paenibacillus mucilaginosus TKU032 conversion and its application in the preparation of bioactive chitosan oligosaccharides (2019), Mar. Drugs, 17, 217.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.132 food industry chitosanase from Paenibacillus mucilaginosus TKU032 may have potential applications in production of bioactive chitosan oligosaccharides for the food and pharmaceutical industries Paenibacillus mucilaginosus
3.2.1.132 pharmacology chitosanase from Paenibacillus mucilaginosus TKU032 may have potential applications in production of bioactive chitosan oligosaccharides for the food and pharmaceutical industries Paenibacillus mucilaginosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Ba2+ 5 mM, 38% loss of activity Paenibacillus mucilaginosus
3.2.1.132 Ca2+ 5 mM, 23% loss of activity Paenibacillus mucilaginosus
3.2.1.132 Cu2+ 5 mM, 26% loss of activity Paenibacillus mucilaginosus
3.2.1.132 EDTA 5 mM, 16% loss of activity Paenibacillus mucilaginosus
3.2.1.132 Fe2+ 5 mM, 36% loss of activity Paenibacillus mucilaginosus
3.2.1.132 Mg2+ 5 mM, 16% loss of activity Paenibacillus mucilaginosus
3.2.1.132 Na+ 5 mM, 8% loss of activity Paenibacillus mucilaginosus
3.2.1.132 Zn2+ 5 mM, 23% loss of activity Paenibacillus mucilaginosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 59000
-
 SDS-PAGE Paenibacillus mucilaginosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Paenibacillus mucilaginosus
-
-
-
3.2.1.132 Paenibacillus mucilaginosus TKU032
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Paenibacillus mucilaginosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.132 culture condition medium containing shrimp heads as the sole carbon and nitrogen source Paenibacillus mucilaginosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 6.03
-
 pH 7, 37°C Paenibacillus mucilaginosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 cellulose + H2O
-
 Paenibacillus mucilaginosus ?
-
 ?
3.2.1.132 cellulose + H2O
-
 Paenibacillus mucilaginosus TKU032 ?
-
 ?
3.2.1.132 chitin + H2O
-
 Paenibacillus mucilaginosus ?
-
 ?
3.2.1.132 chitin + H2O
-
 Paenibacillus mucilaginosus TKU032 ?
-
 ?
3.2.1.132 chitosan + H2O excellent activity on colloidal chitosan (338.74%), followed by chitosan powder (185.38%) Paenibacillus mucilaginosus ?
-
 ?
3.2.1.132 chitosan + H2O excellent activity on colloidal chitosan (338.74%), followed by chitosan powder (185.38%) Paenibacillus mucilaginosus TKU032 ?
-
 ?
3.2.1.132 additional information no activity with 4-nitrophenyl-N-acetyl-beta-D-glucosaminide Paenibacillus mucilaginosus ?
-
 ?
3.2.1.132 additional information no activity with 4-nitrophenyl-N-acetyl-beta-D-glucosaminide Paenibacillus mucilaginosus TKU032 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 59000, SDS-PAGE Paenibacillus mucilaginosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 TKU032 chitosanase
-
 Paenibacillus mucilaginosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 70
-
-
 Paenibacillus mucilaginosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.132 50 100 50°C: about 50% of maximal activity, 100°C: about 40% of maximal activity Paenibacillus mucilaginosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 6
-
-
 Paenibacillus mucilaginosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 4 9 pH 4.0: about 75% of maximal activity, pH 9.0: about 50% of maximal activity Paenibacillus mucilaginosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 4
-
 30 min, below pH 4 activity disappears completely Paenibacillus mucilaginosus
3.2.1.132 4 8 30 min, over 80% of activity is retained Paenibacillus mucilaginosus
3.2.1.132 9
-
 30 min, 50% of the activity is retained Paenibacillus mucilaginosus