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Literature summary extracted from

  • Cuebas-Irizarry, M.F.; Irizarry-Caro, R.A.; Lopez-Morales, C.; Badillo-Rivera, K.M.; Rodriguez-Minguela, C.M.; Montalvo-Rodriguez, R.
    Cloning and molecular characterization of an alpha-glucosidase (MalH) from the halophilic archaeon Haloquadratum walsbyi (2017), Life, 7, E46 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 expressed in Escherichia coli Rosetta cells Haloquadratum walsbyi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 cytoplasm
-
 Haloquadratum walsbyi 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.20 K+ enzyme activity is the highest in the presence of 3-4 M KCl Haloquadratum walsbyi
3.2.1.20 Na+ enzyme activity is the highest in the presence of 3 M NaCl Haloquadratum walsbyi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Haloquadratum walsbyi
-
-
-
3.2.1.20 Haloquadratum walsbyi DSM 16854
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 Ni-NTA column chromatography Haloquadratum walsbyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Haloquadratum walsbyi 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Haloquadratum walsbyi DSM 16854 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.20 additional information no activity with 4-nitrophenyl alpha-D-xylopyranoside Haloquadratum walsbyi ?
-
 ?
3.2.1.20 additional information no activity with 4-nitrophenyl alpha-D-xylopyranoside Haloquadratum walsbyi DSM 16854 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 ? x * 87000, SDS-PAGE Haloquadratum walsbyi

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 MalH
-
 Haloquadratum walsbyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 40
-
-
 Haloquadratum walsbyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 6
-
-
 Haloquadratum walsbyi