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Literature summary extracted from

  • Chen, J.H.; Xu, W.; Sheppard, D.N.
    Altering intracellular pH reveals the kinetic basis of intraburst gating in the CFTR Cl- channel (2017), J. Physiol., 595, 1059-1076 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.6 mutant enzymes are expressed in HeLa cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.6.1.6 D572N the mutation disrupts the interaction of ATP with ATP-binding site 1 Homo sapiens
5.6.1.6 G1349D the mutation is associated with cystic fibrosis and greatly disturbs enzyme intraburst gating Homo sapiens
5.6.1.6 K464A the mutation disrupts the interaction of ATP with ATP-binding site 1 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.6 ATP + H2O + closed Cl- channel Homo sapiens
-
 ADP + phosphate + open Cl- channel
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.6 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.6 ATP + H2O + closed Cl- channel
-
 Homo sapiens ADP + phosphate + open Cl- channel
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.6 CFTR
-
 Homo sapiens
5.6.1.6 cystic fibrosis transmembrane conductance regulator
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.6.1.6 5.8 8.8 when compared with the control (pH 7.3), acidifying pH to 6.3 or alkalinizing pH to 8.3 and 8.8 causes small reductions in the open-time constant of wild type enzyme. By contrast, the fast closed-time constant which describes the short-lived closures that interrupt open bursts, is greatly increased at pH 5.8 and 6.3 Homo sapiens