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Literature summary extracted from

  • Fratebianchi, D.; Cavello, I.A.; Cavalitto, S.F.
    Purification and biochemical and kinetic properties of an endo-polygalacturonase from the industrial fungus Aspergillus sojae (2017), J. Mol. Microbiol. Biotechnol., 27, 102-109 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 Hg2+ complete inhibition at 1 mM Aspergillus sojae
3.2.1.15 additional information not affected by 2-mercaptoethanol, 15% (v/v) ethanol or 100 mg/l SO2 Aspergillus sojae
3.2.1.15 trigalacturonate
-
 Aspergillus sojae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.15 additional information not affected by Ca2+, K+, Mg2+, and Zn2+ Aspergillus sojae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Aspergillus sojae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 Q Sepharose column chromatography, SP Sepharose column chromatography, and Superdex 75 gel filtration Aspergillus sojae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 citrus pectin + H2O
-
 Aspergillus sojae ?
-
 ?
3.2.1.15 lemon protopectin + H2O
-
 Aspergillus sojae ?
-
 ?
3.2.1.15 polygalacturonate + H2O
-
 Aspergillus sojae trigalacturonate + digalactuornate + D-galacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 ? x * 47000, SDS-PAGE Aspergillus sojae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 endo-polygalacturonase
-
 Aspergillus sojae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 50
-
 the enzyme presents a half-life of 2 h at 50°C. The enzyme is fully thermostable up to 45°C, whereas 25% of the initial activity is retained after 210 min of incubation at 50°C Aspergillus sojae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 3.5
-
 with citrus pectin as substrate Aspergillus sojae
3.2.1.15 4.3
-
 with lemon protopectin as substrate Aspergillus sojae
3.2.1.15 5
-
 with polygalacturonate as substrate Aspergillus sojae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.15 1 6.5 after 2 h of incubation, the purified enzyme retains more than 75% of its initial activity between the pH values of 2.0 and 6.5, and shows up to 55% residual activity within a very low pH interval (1.0-2.0). After 24 h in the pH range of 1.0-3.0 the enzyme retains 35% of its initial activity at pH 1.0 and more than 75% from pH 1.5 onwards Aspergillus sojae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.15 0.6
-
 trigalacturonate at pH 5.0 and 20°C Aspergillus sojae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.15 Aspergillus sojae isoelectric focusing
-
 4.2