Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • De Lise, F.; Mensitieri, F.; Tarallo, V.; Ventimiglia, N.; Vinciguerra, R.; Tramice, A.; Marchetti, R.; Pizzo, E.; Notomista, E.; Cafaro, V.; Molinaro, A.; Birolo, L.; Di Donato, A.; Izzo, V.
    RHA-P Isolation, expression and characterization of a bacterial alpha-L-rhamnosidase from Novosphingobium sp. PP1Y (2016), J. Mol. Catal. B, 134, 136-147 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.40 biotechnology potential use as a biocatalyst for diverse biotechnological applications Novosphingobium sp. PP1Y

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.40 expression in Escherichia coli BL21(DE3) Novosphingobium sp. PP1Y

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 160.2
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 6.9, temperature not specified in the publication Novosphingobium sp. PP1Y

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 101000
-
 gel filtration Novosphingobium sp. PP1Y

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.40 Acetone 10% acetone, 50 mM MOPS, pH 6.9, 6.73% residual activity. Complete loss of activity in 50% acetone Novosphingobium sp. PP1Y
3.2.1.40 DMSO 10% DMSO, in 50 mM MOPS, pH 6.9, 66.29% residual activity. Complete loss of activity in 50% DMSO Novosphingobium sp. PP1Y
3.2.1.40 Ethanol 10% ethanol, 50 mM MOPS, pH 6.9, 66.52% residual activity. Complete loss of activity in 50% ethanol Novosphingobium sp. PP1Y

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Novosphingobium sp. PP1Y F6IEX3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40 expression in Escherichia coli, strain BL21(DE3) Novosphingobium sp. PP1Y

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Novosphingobium sp. PP1Y 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 naringin + H2O
-
 Novosphingobium sp. PP1Y beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 neohesperidin dihydrochalcone + H2O
-
 Novosphingobium sp. PP1Y 3,5-dihydroxy-4-[3-(3-hydroxy-4-methoxyphenyl)propanoyl]phenyl beta-D-glucopyranoside + alpha-L-rhamnose
-
 ?
3.2.1.40 rutin + H2O
-
 Novosphingobium sp. PP1Y isoquercitrin + alpha-L-rhamnose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 monomer
-
 Novosphingobium sp. PP1Y

Synonyms

EC Number Synonyms Comment Organism
3.2.1.40 alpha-RHA
-
 Novosphingobium sp. PP1Y

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 40.9
-
-
 Novosphingobium sp. PP1Y

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.40 32 45 32°C: about 55% of maximal activity, 45°C: about 65% of maximal activity Novosphingobium sp. PP1Y

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 25 40 3 h, stable Novosphingobium sp. PP1Y
3.2.1.40 40
-
 24 h, stable Novosphingobium sp. PP1Y

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 6 7.5
-
 Novosphingobium sp. PP1Y

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.40 7.9 8.8 the enzyme retains 47% and 36% of activity at pH 7.9 and 8.8 Novosphingobium sp. PP1Y