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Literature summary extracted from

  • Homaei, A.
    Purification and biochemical properties of highly efficient alkaline phosphatase from Fenneropenaeus merguiensis brain (2015), J. Mol. Catal. B, 118, 16-22 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 Ca2+ 83% residual activity at 5 mM Penaeus merguiensis
3.1.3.1 Cu2+ complete inhibition at 2 mM Penaeus merguiensis
3.1.3.1 Hg2+ complete inhibition at 2 mM Penaeus merguiensis
3.1.3.1 K+ 90% residual activity at 4 mM Penaeus merguiensis
3.1.3.1 Li+ 83% residual activity at 5 mM Penaeus merguiensis
3.1.3.1 Mg2+ 93% residual activity at 5 mM Penaeus merguiensis
3.1.3.1 Na+ 91% residual activity at 5 mM Penaeus merguiensis
3.1.3.1 Pb2+ complete inhibition at 2 mM Penaeus merguiensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.0003
-
 4-nitrophenyl phosphate at pH 11.0 and 25°C Penaeus merguiensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Ca2+ 125% activity at 2 mM Penaeus merguiensis
3.1.3.1 Mg2+ 123% activity at 1 mM Penaeus merguiensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Penaeus merguiensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1 ammonium sulfate precipitation, DEAE-cellulose column chromatography, DEAE-Sephadex gel filtration, and Sephadex G-200 gel filtration Penaeus merguiensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.1 brain
-
 Penaeus merguiensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O strongest affinity substrate Penaeus merguiensis 4-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 ? x * 70000, SDS-PAGE Penaeus merguiensis

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 ALPase
-
 Penaeus merguiensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 50
-
-
 Penaeus merguiensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.1 30 70 the enzyme retains a high activity (more than 50%) in a wide temperature range from about 30-70°C. About 35% of its maximum phosphatase activity is retained at low (25°C) and very high (80°C) temperatures Penaeus merguiensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.1 95
-
 4-nitrophenyl phosphate at pH 11.0 and 25°C Penaeus merguiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 11
-
-
 Penaeus merguiensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.1 6 12 the enzyme retains a high activity (more than 50%) in the pH range from pH 6.0-12.0 Penaeus merguiensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.1 316600
-
 4-nitrophenyl phosphate at pH 11.0 and 25°C Penaeus merguiensis