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Literature summary extracted from

  • Xiong, Z.J.; Huang, J.; Poda, G.; Pomes, R.; Prive, G.G.
    Structure of human acid sphingomyelinase reveals the role of the saposin domain in activating substrate hydrolysis (2016), J. Mol. Biol., 428, 3026-3042 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 additional information the enzyme with a a built-in N-terminal saposin domain does not require an external saposin activator protein Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.12 vapor diffusion method, using 0.2 M sodium acetate, 20% (w/v) polyethylene glycol 8000, 0.1 M sodium cacodylate, pH 6.0 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.12 D278A inactive Homo sapiens
3.1.4.12 H319Y inactive Homo sapiens
3.1.4.12 H425R inactive Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 lysosome
-
 Homo sapiens 5764
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Zn2+ binuclear zinc center Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Homo sapiens
-
 ceramide + phosphocholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Homo sapiens P17405
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.4.12 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 ammonium sulfate precipitation, HiTrap phenyl column chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
 Homo sapiens ceramide + phosphocholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 acid sphingomyelinase
-
 Homo sapiens
3.1.4.12 ASM
-
 Homo sapiens
3.1.4.12 SMPD1
-
 Homo sapiens