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Literature summary extracted from

  • Zuo, Z.; Rodgers, C.J.; Mikheikin, A.L.; Trakselis, M.A.
    Characterization of a functional DnaG-type primase in archaea implications for a dual-primase system (2010), J. Mol. Biol., 397, 664-676 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.101 expression in Escherichia coli Saccharolobus solfataricus
2.7.7.102 expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.101 E175G complete loss of activity Saccharolobus solfataricus
2.7.7.102 E175G complete loss of activity Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.101 0.085
-
 NTP pH 8.5, 70°C Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.101 Mg2+ or Mn2+, required. Optimum concentration 10 mM Saccharolobus solfataricus
2.7.7.101 Mn2+ or Mg2+, required. Optimum concentration 11 mM Saccharolobus solfataricus
2.7.7.102 Mg2+ or Mn2+, required. Optimum concentration 10 mM Saccharolobus solfataricus
2.7.7.102 Mn2+ or Mg2+, required. Optimum concentration 11 mM Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.101 Saccharolobus solfataricus P95980
-
-
2.7.7.101 Saccharolobus solfataricus ATCC 35092 P95980
-
-
2.7.7.102 Saccharolobus solfataricus Q97Z83 and Q9UWW1 Q97Z83 i. e. small subunit PriS, Q9UWW1 i.e. large subunit PriL
-
2.7.7.102 Saccharolobus solfataricus ATCC 35092 Q97Z83 and Q9UWW1 Q97Z83 i. e. small subunit PriS, Q9UWW1 i.e. large subunit PriL
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.101 from insoluble recombinant aggregates, solubilization by addition of high concentrations of glycerol, polyethylene glycol, or Mn2+, and by incubation at 60°C Saccharolobus solfataricus
2.7.7.102 from insoluble recombinant aggregates, solubilization by addition of high concentrations of glycerol, polyethylene glycol, or Mn2+, and by incubation at 60°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.101 bacteriophage M13 ssDNA + n NTP
-
 Saccharolobus solfataricus bacteriophage M13 ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate
-
 ?
2.7.7.101 bacteriophage M13 ssDNA + n NTP
-
 Saccharolobus solfataricus ATCC 35092 bacteriophage M13 ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate
-
 ?
2.7.7.102 bacteriophage M13 ssDNA + n NTP
-
 Saccharolobus solfataricus bacteriophage M13 ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate
-
 ?
2.7.7.102 bacteriophage M13 ssDNA + n NTP
-
 Saccharolobus solfataricus ATCC 35092 bacteriophage M13 ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.101 Sso0079
-
 Saccharolobus solfataricus
2.7.7.102 Sso0079
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.101 70
-
-
 Saccharolobus solfataricus
2.7.7.102 70
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.101 50
-
 poor activity below Saccharolobus solfataricus
2.7.7.102 50
-
 poor activity below Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.101 0.00055
-
 NTP pH 8.5, 70°C Saccharolobus solfataricus
2.7.7.102 0.00013
-
 NTP pH 8.5, 70°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.101 8.5
-
 50 mM Tris-HCl buffer Saccharolobus solfataricus
2.7.7.102 8.5
-
 50 mM Tris-HCl buffer Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.101 0.006
-
 NTP pH 8.5, 70°C Saccharolobus solfataricus
2.7.7.102 0.00041
-
 NTP pH 8.5, 70°C Saccharolobus solfataricus