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Literature summary extracted from

  • Lao-Sirieix, S.; Bell, S.
    The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3'-terminal nucleotidyl transferase activities (2004), J. Mol. Biol., 344, 1251-1263 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.102 coexpression of both subunits in Saccharomyces cerevisiae and Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.102 D101 catalytic site mutant, complete loss of activity Saccharolobus solfataricus
2.7.7.102 D103 catalytic site mutant, complete loss of activity Saccharolobus solfataricus
2.7.7.102 N175 residue involved in DNA-binding. Mutant accumulates dinucleotides, no other products are formed Saccharolobus solfataricus
2.7.7.102 R176 residue involved in DNA binding. Mutant accumulates dinucleotides, no other products are formed Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.102 Saccharolobus solfataricus Q97Z83 and Q9UWW1 Q97Z83 i.e. small subunit PriS, Q9UWW1 i.e. large subunit PriL
-
2.7.7.102 Saccharolobus solfataricus DSM 1617 Q97Z83 and Q9UWW1 Q97Z83 i.e. small subunit PriS, Q9UWW1 i.e. large subunit PriL
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.102 M13 ssDNA + n dNTP
-
 Saccharolobus solfataricus M13 ssDNA/pppdN(pdN)n-1 + (n-1) diphosphate
-
 ?
2.7.7.102 M13 ssDNA + n dNTP
-
 Saccharolobus solfataricus DSM 1617 M13 ssDNA/pppdN(pdN)n-1 + (n-1) diphosphate
-
 ?
2.7.7.102 M13 ssDNA + n NTP
-
 Saccharolobus solfataricus M13 ssDNA/pppN(pN)n-1 + (n-1) diphosphate
-
 ?
2.7.7.102 M13 ssDNA + n NTP
-
 Saccharolobus solfataricus DSM 1617 M13 ssDNA/pppN(pN)n-1 + (n-1) diphosphate
-
 ?
2.7.7.102 phage X174 ssDNA + n NTP
-
 Saccharolobus solfataricus phage X174 ssDNA/pppN(pN)n-1 + (n-1) diphosphate
-
 ?
2.7.7.102 phage X174 ssDNA + n NTP
-
 Saccharolobus solfataricus DSM 1617 phage X174 ssDNA/pppN(pN)n-1 + (n-1) diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.102 ? x * 35700, subunit PriL, plus x * 37600, subunit PriS Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
2.7.7.102 physiological function PriSL is capable of utilising both ribonucleotides and deoxyribonucleotides for primer synthesis in the presence of natural, or synthetic, single-stranded DNA. Products range from dinucleotides to DNA molecules in excess of 7 kb and RNA up to 1 kb in length. PriSL has a significantly higher affinity for ribonucleotides than for deoxyribonucleotides Saccharolobus solfataricus