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Literature summary extracted from

  • Jeong, W.S.; Seo, D.H.; Jung, J.H.; Jung, D.H.; Lee, D.W.; Park, Y.S.; Park, C.
    Enzymatic characteristics of a highly thermostable beta-(1-4)-glucanase from Fervidobacterium islandicum AW-1 (KCTC 4680) (2017), J. Microbiol. Biotechnol., 27, 271-276 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.74 biofuel production the enzyme can be used for hydrolysis of cellulosic and hemicellulosic biomass substrates for biofuel production Fervidobacterium islandicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.74 expressed in Escherichia coli BL21(DE3) cells Fervidobacterium islandicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.74 Cd2+ 28.6% residual activity at 5 mM Fervidobacterium islandicum
3.2.1.74 Co2+ 90% residual activity at 5 mM Fervidobacterium islandicum
3.2.1.74 Cu2+ 34.7% residual activity at 5 mM Fervidobacterium islandicum
3.2.1.74 EDTA 17.6% residual activity at 5 mM Fervidobacterium islandicum
3.2.1.74 Fe2+ 69.1% residual activity at 5 mM Fervidobacterium islandicum
3.2.1.74 Na+ 92.8% residual activity at 5 mM Fervidobacterium islandicum
3.2.1.74 SDS 45.7% residual activity at 0.5% (w/v) Fervidobacterium islandicum
3.2.1.74 Triton X-100 98.6% residual activity at 0.5% (w/v) Fervidobacterium islandicum
3.2.1.74 Zn2+ 65.1% residual activity at 5 mM Fervidobacterium islandicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.74 Ba2+ 122% activity at 5 mM Fervidobacterium islandicum
3.2.1.74 Ca2+ 105.7% activity at 5 mM Fervidobacterium islandicum
3.2.1.74 Mn2+ 108.3% activity at 5 mM Fervidobacterium islandicum
3.2.1.74 additional information not affected by Mg2+ Fervidobacterium islandicum
3.2.1.74 Ni2+ 116.2% activity at 5 mM Fervidobacterium islandicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.74 Fervidobacterium islandicum
-
 KCTC 4680
-
3.2.1.74 Fervidobacterium islandicum AW-1
-
 KCTC 4680
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.74 Ni-NTA column chromatography Fervidobacterium islandicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.74 4-nitrophenyl cellobioside + H2O 2756% activity compared to carboxymethyl cellulose Fervidobacterium islandicum 4-nitrophenol + cellobiose
-
 ?
3.2.1.74 4-nitrophenyl cellobioside + H2O 2756% activity compared to carboxymethyl cellulose Fervidobacterium islandicum AW-1 4-nitrophenol + cellobiose
-
 ?
3.2.1.74 avicel + H2O very low activity Fervidobacterium islandicum ?
-
 ?
3.2.1.74 avicel + H2O very low activity Fervidobacterium islandicum AW-1 ?
-
 ?
3.2.1.74 barley beta-D-glucan + H2O strongest activity (15525% activity compared to carboxymethyl cellulose) Fervidobacterium islandicum D-glucose
-
 ?
3.2.1.74 cellohexaose + H2O
-
 Fervidobacterium islandicum cellobiose + cellotriose
-
 ?
3.2.1.74 cellopentaose + H2O
-
 Fervidobacterium islandicum cellobiose + cellotriose
-
 ?
3.2.1.74 cellotetraose + H2O
-
 Fervidobacterium islandicum 2 cellobiose
-
 ?
3.2.1.74 cellotetraose + H2O
-
 Fervidobacterium islandicum AW-1 2 cellobiose
-
 ?
3.2.1.74 galactomannan + H2O 7363% activity compared to carboxymethyl cellulose Fervidobacterium islandicum ?
-
 ?
3.2.1.74 galactomannan + H2O 7363% activity compared to carboxymethyl cellulose Fervidobacterium islandicum AW-1 ?
-
 ?
3.2.1.74 hydroxyethyl cellulose + H2O 220% activity compared to carboxymethyl cellulose Fervidobacterium islandicum ?
-
 ?
3.2.1.74 additional information no activity with soluble starch, laminarin, and mannan Fervidobacterium islandicum ?
-
 ?
3.2.1.74 additional information no activity with soluble starch, laminarin, and mannan Fervidobacterium islandicum AW-1 ?
-
 ?
3.2.1.74 oat spelt xylan + H2O 57% activity compared to carboxymethyl cellulose Fervidobacterium islandicum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.74 ? x * 37000, SDS-PAGE Fervidobacterium islandicum
3.2.1.74 ? x * 41400, calculated from amino acid sequence Fervidobacterium islandicum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.74 beta-(1-4)-glucanase
-
 Fervidobacterium islandicum
3.2.1.74 FiG
-
 Fervidobacterium islandicum
3.2.1.74 NA23_08975
-
 Fervidobacterium islandicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.74 80 90
-
 Fervidobacterium islandicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.74 50 100 about 30% activity at 50°C about 55% activity at 60°C, about 80% activity at 70°C, 100% activity at 80-90°C, about 30% activity at 100°C Fervidobacterium islandicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.74 70 90 the hydrolyzing activity is not diminished up to 10 days at 70°C or 75°C. The half-lives of the recombinant enzyme are about 200 and 113 h at 80°C and 85°C, respectively. The enzyme activity is drastically reduced at 90°C Fervidobacterium islandicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.74 5
-
-
 Fervidobacterium islandicum