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Literature summary extracted from

  • Tan, H.; Tang, J.; Li, X.; Liu, T.; Miao, R.; Huang, Z.; Wang, Y.; Gan, B.; Peng, W.
    Biochemical characterization of a psychrophilic phytase from an artificially cultivable morel Morchella importuna (2017), J. Microbiol. Biotechnol., 27, 2180-2189 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.8 additional information no effect by EDTA and EGTA at up to 20 mM Morchella importuna

Application

EC Number Application Comment Organism
3.1.3.8 agriculture since the enzyme degrades phytate in feed materials efficiently under low temperature and weak acidic conditions, which are common for aquacultural application, it might be a promising candidate as a feed additive enzyme Morchella importuna

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 gene phyA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression in Pichia pastoris strain GS115 Morchella importuna

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Al3+ slight inhibition Morchella importuna
3.1.3.8 Ba2+
-
 Morchella importuna
3.1.3.8 Cu2+ slight inhibition Morchella importuna
3.1.3.8 Fe2+ slight inhibition Morchella importuna
3.1.3.8 Fe3+
-
 Morchella importuna
3.1.3.8 Hg2+
-
 Morchella importuna
3.1.3.8 Mn2+ slight inhibition Morchella importuna
3.1.3.8 Pb2+
-
 Morchella importuna
3.1.3.8 SDS 90% inhibition at 1%, complete inhibition at 10%, 10% inhibition at 0.1% Morchella importuna
3.1.3.8 Urea 40% inhibition at 100 mM, complete inhibition at 5 M Morchella importuna
3.1.3.8 Zn2+ slight inhibition Morchella importuna

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 extracellular no signal peptide is identified Morchella importuna
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 61089
-
 mass spectrometry Morchella importuna

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O Morchella importuna
-
 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O Morchella importuna SCYDJ1A1
-
 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Morchella importuna A0A2H4W727
-
-
3.1.3.8 Morchella importuna SCYDJ1A1 A0A2H4W727
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.8 fruit body
-
 Morchella importuna
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 2384.6
-
 purified enzyme, pH 6.5, 25°C Morchella importuna

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 additional information the enzyme also shows moderate activity with 4-nitrophenyl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1,6-bisphosphate, and glucose 6-phosphate, and low activity with AMP, ADP, and ATP. The enzyme cleaves phytate in soybean meal, rapeseed meal, and corn meal Morchella importuna ?
-
 ?
3.1.3.8 additional information the enzyme also shows moderate activity with 4-nitrophenyl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1,6-bisphosphate, and glucose 6-phosphate, and low activity with AMP, ADP, and ATP. The enzyme cleaves phytate in soybean meal, rapeseed meal, and corn meal Morchella importuna SCYDJ1A1 ?
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Morchella importuna 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Morchella importuna SCYDJ1A1 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 monomer 1 * 61090, sequence calculation, 1 * 61089, mass spectrometry, 1 * 60000, SDS-PAGE Morchella importuna

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 MiPhyA
-
 Morchella importuna
3.1.3.8 PhyA
-
 Morchella importuna

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 25
-
-
 Morchella importuna

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8
-
 30 over 85% of maximal activity at 15-25°C, maximal activity at 25°C, sharp drop inactivity at above 30°C, over 50% of maximal activity at 0-5°C Morchella importuna

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 25
-
 purified enzyme, pH 6.5, 180 min, 50% activity remaining Morchella importuna
3.1.3.8 45
-
 purified enzyme, pH 6.5, 50% activity remaining after 120 min, 20% after 180 min Morchella importuna
3.1.3.8 65
-
 purified enzyme, pH 6.5, 50% activity remaining after about 20 min, 10% after 60 min, inactivation after 120 min Morchella importuna

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 6.5
-
-
 Morchella importuna

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 2 7.5 activity range, profile overview. sharp drop in activity above pH 6.5 Morchella importuna

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 4
-
 purified enzyme, 25°C, about 50% activity remaining after about 50 min, inactivation after 180 min Morchella importuna
3.1.3.8 6.5
-
 purified enzyme, 25°C, 240 min, 40% activity remaining Morchella importuna
3.1.3.8 11
-
 purified enzyme, 25°C, about 50% activity remaining after about 50 min, inactivation after 180 min Morchella importuna

General Information

EC Number General Information Comment Organism
3.1.3.8 evolution the enzyme belongs to the histidine acid phosphatase (HAP) family, it possesses typical conserved motifs of HAP phytases: RHGERFP in AA125-131 and HD in AA394-395 Morchella importuna