EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.165 | expressed in Pichia pastoris GS115 cells and Escherichia coli Rosetta (DE3) pLysS cells | Aspergillus oryzae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.165 | F769W | the mutation significantly enhances the specific activity and hydrolysis velocity compared to the wild type enzyme | Aspergillus oryzae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.165 | Ag+ | 47.9% residual activity at 5 mg/ml, complete inhibition at 10 mg/ml | Aspergillus oryzae | |
3.2.1.165 | Ca2+ | 76.8% residual activity at 10 mg/ml | Aspergillus oryzae | |
3.2.1.165 | Co2+ | 4.8% residual activity at 10 mg/ml | Aspergillus oryzae | |
3.2.1.165 | Cu2+ | 19.3% residual activity at 10 mg/ml | Aspergillus oryzae | |
3.2.1.165 | EDTA | 65% residual activity at 1 mg/ml, complete inhibition at 5 mg/ml | Aspergillus oryzae | |
3.2.1.165 | Fe2+ | 16.1% residual activity at 10 mg/ml | Aspergillus oryzae | |
3.2.1.165 | K+ | 84.6% residual activity at 10 mg/ml | Aspergillus oryzae | |
3.2.1.165 | Mg2+ | 55.8% residual activity at 10 mg/ml | Aspergillus oryzae | |
3.2.1.165 | Mn2+ | 2.5% residual activity at 5 mg/ml, complete inhibition at 10 mg/ml | Aspergillus oryzae | |
3.2.1.165 | Ni2+ | 82.9% residual activity at 10 mg/ml | Aspergillus oryzae | |
3.2.1.165 | Zn2+ | 55.8% residual activity at 10 mg/ml | Aspergillus oryzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.165 | 18.4 | - |
colloidal chitosan | wild type enzyme, at pH 5.5 and 50°C | Aspergillus oryzae | |
3.2.1.165 | 32.9 | - |
colloidal chitosan | mutant enzyme F769W, at pH 5.5 and 50°C | Aspergillus oryzae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.165 | additional information | not influenced by Ba2+ | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.165 | Aspergillus oryzae | W8E1X9 | - |
- |
3.2.1.165 | Aspergillus oryzae FL402 | W8E1X9 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.165 | glycoprotein | - |
Aspergillus oryzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.165 | colloidal chitosan + H2O | - |
Aspergillus oryzae | beta-D-glucosamine + ? | - |
? | |
3.2.1.165 | colloidal chitosan + H2O | - |
Aspergillus oryzae FL402 | beta-D-glucosamine + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.165 | ? | x * 100000, SDS-PAGE | Aspergillus oryzae |
3.2.1.165 | ? | x * 99200, calculated from amino acid sequence | Aspergillus oryzae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.165 | CsxA | - |
Aspergillus oryzae |
3.2.1.165 | exo-beta-D-glucosaminidase | - |
Aspergillus oryzae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.165 | 50 | - |
- |
Aspergillus oryzae |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.165 | 40 | 60 | the recombinant enzyme expressed in Escherichia coli shows more than 45% activity between 40 and 60°C. The recombinant enzyme expressed in Pichia pastoris shows more than 70% activity between 40 and 60°C | Aspergillus oryzae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.165 | 50 | - |
the recombinant enzyme from Pichia pastoris remains about 80% activity after 25 h at 50°C, while the recombinant enzyme from Escherichia coli is completely inactive (0.48% residual activity) after 3 h at 50°C | Aspergillus oryzae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.165 | 23.8 | - |
colloidal chitosan | wild type enzyme, at pH 5.5 and 50°C | Aspergillus oryzae | |
3.2.1.165 | 102.6 | - |
colloidal chitosan | mutant enzyme F769W, at pH 5.5 and 50°C | Aspergillus oryzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.165 | 5.5 | - |
- |
Aspergillus oryzae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.165 | 4 | 8 | the recombinant enzyme expressed in Escherichia coli shows about 40% activity at pH 4.0, about 80% activity at pH 5.0, 100% activity at pH 5.5, about 90% activity at pH 6.0, and about 20% activity at pH 8.0. The recombinant enzyme expressed in Pichia pastoris shows about 70% activity at pH 4.0, about 90% activity at pH 5.0, 100% activity at pH 5.5, about 95% activity at pH 6.0, and about 20% activity at pH 8.0 | Aspergillus oryzae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.165 | 1.29 | - |
colloidal chitosan | wild type enzyme, at pH 5.5 and 50°C | Aspergillus oryzae | |
3.2.1.165 | 3.11 | - |
colloidal chitosan | mutant enzyme F769W, at pH 5.5 and 50°C | Aspergillus oryzae |