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Literature summary extracted from

  • Zhou, H.; Li, X.; Guo, M.; Xu, Q.; Cao, Y.; Qiao, D.; Cao, Y.; Xu, H.
    Secretory expression and characterization of an acidic endo-polygalacturonase gene from Aspergillus niger SC323 in Saccharomyces cerevisiae (2015), J. Microbiol. Biotechnol., 25, 999-1006 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.15 expressed in Saccharomyces cerevisiae strain EBY100 Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 Ba2+ about 92% residual activity at 1 mM Aspergillus niger
3.2.1.15 Co2+ about 75% residual activity at 1 mM Aspergillus niger
3.2.1.15 Mn2+ about 55% residual activity at 1 mM Aspergillus niger
3.2.1.15 Ni2+ about 82% residual activity at 1 mM Aspergillus niger
3.2.1.15 Pb2+ about 38% residual activity at 1 mM Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.15 Ca2+ about 125% activity at 1 mM Aspergillus niger
3.2.1.15 Cu2+ about 123% activity at 1 mM Aspergillus niger
3.2.1.15 Fe2+ about 115% activity at 1 mM Aspergillus niger
3.2.1.15 K+ about 115% activity at 1 mM Aspergillus niger
3.2.1.15 Li+ about 110% activity at 1 mM Aspergillus niger
3.2.1.15 Mg2+ about 120% activity at 1 mM Aspergillus niger
3.2.1.15 Na+ about 120% activity at 1 mM Aspergillus niger
3.2.1.15 Zn2+ about 115% activity at 1 mM Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Aspergillus niger A0A0B4UL66
-
-
3.2.1.15 Aspergillus niger SC323 A0A0B4UL66
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 ammonium sulfate precipitation and ENrich SEC 650 gel filtration Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 pectin + H2O
-
 Aspergillus niger galacturonic acid + digalacturonic acid + trigalacturonic acid
-
 ?
3.2.1.15 pectin + H2O
-
 Aspergillus niger SC323 galacturonic acid + digalacturonic acid + trigalacturonic acid
-
 ?
3.2.1.15 polygalacturonate + H2O
-
 Aspergillus niger oligogalacturonates
-
 ?
3.2.1.15 polygalacturonate + H2O
-
 Aspergillus niger SC323 oligogalacturonates
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 ? x * 45000, SDS-PAGE Aspergillus niger
3.2.1.15 ? x * 38800, calculated from amino acid sequence Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 endo-pgaA
-
 Aspergillus niger
3.2.1.15 endo-polygalacturonase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 50
-
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.15 30 60 more than 60% activity between 30 and 60°C Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 35 70 the enzyme retains more than 50% of its activity after incubation at 35-70°C for 60 min, but no activity remains after incubation at 70°C for 2 h Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 5
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.15 3 6 more than 60% activity between pH 3.0 and 6.0 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.15 3 6 the enzyme shows good stability within the pH range of 3.0-6.0 for different incubation times and over 65% of activity is retained Aspergillus niger