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Literature summary extracted from

  • Cherdvorapong, V.; Fujiki, H.; Suyotha, W.; Takeda, Y.; Yano, S.; Takagi, K.; Wakayama, M.
    Enzymatic and molecular characterization of alpha-1,3-glucanase (AglST2) from Streptomyces thermodiastaticus HF3-3 and its relation with alpha-1,3-glucanase HF65 (AglST1) (2018), J. Gen. Appl. Microbiol., 65, 18-25 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.59 NaCl the enzyme activity is significantly stimulated in the presence of 5-20% (w/v) NaCl (140% activity at 15% (w/v)) Streptomyces thermodiastaticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.59 expressed in Escherichia coli BL21(DE3) cells Streptomyces thermodiastaticus

General Stability

EC Number General Stability Organism
3.2.1.59 the enzyme retains full activity when treated with 20% (w/v) NaCl for 1 h Streptomyces thermodiastaticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.59 Ag+ 57% residual activity at 1 mM Streptomyces thermodiastaticus
3.2.1.59 Cu2+ 44% residual activity at 1 mM Streptomyces thermodiastaticus
3.2.1.59 EDTA 1-50 mM EDTA decreases the relative activity by almost 50% Streptomyces thermodiastaticus
3.2.1.59 Fe2+ 55% residual activity at 1 mM Streptomyces thermodiastaticus
3.2.1.59 additional information not inhibited by dithiothreitol Streptomyces thermodiastaticus
3.2.1.59 Ni2+ 64% residual activity at 1 mM Streptomyces thermodiastaticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.59 Co2+ 140% activity at 1 mM Streptomyces thermodiastaticus
3.2.1.59 Mn2+ 140% activity at 1 mM Streptomyces thermodiastaticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.59 Streptomyces thermodiastaticus
-
-
-
3.2.1.59 Streptomyces thermodiastaticus HF3-3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.59 DEAE-cellufine A500 column chromatography and HiTrap Q column chromatography Streptomyces thermodiastaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.59 alpha-1,3-glucan + H2O 100% activity Streptomyces thermodiastaticus ?
-
 ?
3.2.1.59 alpha-1,3-glucan + H2O 100% activity Streptomyces thermodiastaticus HF3-3 ?
-
 ?
3.2.1.59 additional information no activity with cellulose, xylan, dextran, amylopectin, laminarin, and amylose Streptomyces thermodiastaticus ?
-
 ?
3.2.1.59 additional information no activity with cellulose, xylan, dextran, amylopectin, laminarin, and amylose Streptomyces thermodiastaticus HF3-3 ?
-
 ?
3.2.1.59 nigeran + H2O 10% activity compared to alpha-1,3-glucan Streptomyces thermodiastaticus ?
-
 ?
3.2.1.59 nigeran + H2O 10% activity compared to alpha-1,3-glucan Streptomyces thermodiastaticus HF3-3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.59 ? x * 91000, SDS-PAGE Streptomyces thermodiastaticus
3.2.1.59 ? x * 76860, calculated from amino acid sequence Streptomyces thermodiastaticus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.59 AglST2
-
 Streptomyces thermodiastaticus
3.2.1.59 alpha-1,3-glucanase HF90
-
 Streptomyces thermodiastaticus
3.2.1.59 mutanase
-
 Streptomyces thermodiastaticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.59 60
-
-
 Streptomyces thermodiastaticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.59 65
-
 the residual activity of the enzyme is more than 80% after incubation at 65°C for 1 h Streptomyces thermodiastaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.59 5.5
-
-
 Streptomyces thermodiastaticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.59 5 7 the enzyme retains 80% of the original activity over a pH range of 5.0-7.0, however, the activity gradually decreases at a pH above 7.5 Streptomyces thermodiastaticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.59 4.5 7 the residual activity of the enzyme is more than 80% after incubation at pH 4.5-7.0 for 1 h Streptomyces thermodiastaticus