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Literature summary extracted from

  • Tagad, C.K.; Sabharwal, S.G.
    Purification and characterization of acid phosphatase from Macrotyloma uiflorum seeds (2018), J. Food Sci. Technol., 55, 313-320 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Ag+
-
 Macrotyloma uniflorum
3.1.3.2 Hg2+
-
 Macrotyloma uniflorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.934
-
 4-nitrophenyl phosphate at pH 5.0 and 50°C Macrotyloma uniflorum
3.1.3.2 5
-
 ATP at pH 5.0 and 50°C Macrotyloma uniflorum
3.1.3.2 8.7
-
 ADP at pH 5.0 and 50°C Macrotyloma uniflorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 EDTA
-
 Macrotyloma uniflorum
3.1.3.2 Mg2+ enzyme activity is slightly increased in the presence of Mg2+ ions Macrotyloma uniflorum
3.1.3.2 additional information K+, Ca2+ and Na+ have no effect on the enzyme activity Macrotyloma uniflorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 55000
-
 gel filtration Macrotyloma uniflorum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Macrotyloma uniflorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 UNOsphere-S column chromatography and Sephadex G-100 gel filtration Macrotyloma uniflorum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 seed
-
 Macrotyloma uniflorum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O
-
 Macrotyloma uniflorum 1-naphthol + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O high activity Macrotyloma uniflorum 4-nitrophenol + phosphate
-
 ?
3.1.3.2 ADP + H2O high activity Macrotyloma uniflorum AMP + phosphate
-
 ?
3.1.3.2 ATP + H2O high activity Macrotyloma uniflorum ADP + phosphate
-
 ?
3.1.3.2 beta-glycerophosphate + H2O
-
 Macrotyloma uniflorum glycerol + phosphate
-
 ?
3.1.3.2 D-fructose 6-phosphate + H2O low activity Macrotyloma uniflorum D-fructose + phosphate
-
 ?
3.1.3.2 D-glucose 1-phosphate + H2O low activity Macrotyloma uniflorum D-glucose + phosphate
-
 ?
3.1.3.2 D-glucose 6-phosphate + H2O low activity Macrotyloma uniflorum D-glucose + phosphate
-
 ?
3.1.3.2 phenyl phosphate + H2O high activity Macrotyloma uniflorum phenol + phosphate
-
 ?
3.1.3.2 pyridoxyl 5'-phosphate + H2O low activity Macrotyloma uniflorum pyridoxal + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 heterodimer 1 * 28241 + 1 * 27093, MALDI-TOF mass spectrometry Macrotyloma uniflorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 50
-
-
 Macrotyloma uniflorum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.2 20 70 more than 50% activity between 20 and 70°C Macrotyloma uniflorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 55 80 the enzyme is stable up to 55°C. Further heating of the enzyme results in about 45% loss in enzyme activity at 60°C with a total loss in activity at 80°C Macrotyloma uniflorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5
-
-
 Macrotyloma uniflorum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 3 6 more than 60% activity between pH 3.0 and 6.0 Macrotyloma uniflorum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.2 4 6.5 the enzyme is stable between pH 4.0 and 6.5 Macrotyloma uniflorum