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Literature summary extracted from

  • Li, B.; Duan, D.; Wang, J.; Li, H.; Zhang, X.; Zhao, B.
    Improving phospholipase D activity and selectivity by bio-imprinting-immobilization to produce phosphatidylglycerol (2018), J. Biotechnol., 281, 67-73 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.4.4 synthesis hyperactivation of PLD by bio-imprinting and immobilization by adsorption and precipitation, followed by cross-linking results in increased catalytic activity. The maximum activity of immobilized PLD reaches 166953 U/g protein, compared to 11922 U/mg protein for the free form. The selectivity of PLD is significantly enhanced after immobilization. The yield of phosphatidylglycerol and phosphatidic acid reaches 94.0% and 5.96%, respectively Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Streptomyces sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 commercial preparation
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 11.92
-
 free enzyme, pH 5.5, temperature not specified in the publication Streptomyces sp.
3.1.4.4 166.9
-
 bio-imprinted immobilized enzyme, pH 6.0, temperature not specified in the publication Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 phosphatidylcholine + H2O
-
 Streptomyces sp. choline + phosphatidate
-
 ?
3.1.4.4 phosphatidylethanolamine + H2O
-
 Streptomyces sp. ethanolamine + phosphatidate
-
 ?
3.1.4.4 phosphatidylglycerol + H2O
-
 Streptomyces sp. glycerol + phosphatidate
-
 ?